Abstract:

Abstract： Objective To explore the relationship between the interleukin-6 and Survivin and early embryo arrest. Methods Real time-PCR was used to measure the mRNA expression of IL-6 and Survivin in villus and SP was used to measure the location and expression of the two different kinds of protein in corresponding tissue. ELISA was used to measure the level of IL-6 in serum. Results Real-time PCR and IHC showed that the expression levels of IL-6 and Survivin were significantly lower in experimental group than in control group (P＜0.05). Serum level of IL-6 among the three groups was not statistically significant (P >0.05). Conclusion Proper expressions of IL-6 and Survivin in the villus tissue play a certain role in maintaining early embryo development.

Key words: interleukin-6, Survivin, trophoblast cell , early embryo arrest

摘要：

目的 探讨白介素-6（IL-6）及生存素（Survivin）在胚胎停止发育发病中的可能作用。方法 对2012年7-12月就诊于郑州大学第三附属医院产科门诊30例不明原因胚胎停止发育病例（研究组）和30例正常早孕吸宫流产病例（对照组）的绒毛组织及血清进行研究。用实时荧光定量逆转录聚合酶链式反应技术(Real time-PCR)和免疫组织化学技术(SP法)检测两组绒毛组织中IL-6和Survivin mRNA的表达和蛋白定位、表达强度；用双抗体酶联免疫夹心法（ELISA法）测定IL-6在研究组、对照组和阴性对照组（非孕健康体检者）血清中的浓度。结果 绒毛组织中，IL-6与Survivin在合体及绒毛滋养层细胞中均有表达，Real time-PCR及免疫组化结果均显示IL-6与Survivin在研究组中的表达水平均低于对照组，差异均有统计学意义（P＜0.05）；ELISA法检测研究组中IL-6表达低于对照组和阴性对照组，差异无统计学意义（P＞0.05）。结论 孕早期绒毛组织中IL-6和Survivin的正常表达对维持早期胚胎发育起到一定作用。

关键词: 白介素-6, 生存素, 滋养细胞, 胚胎停育