Abstract:

Abstract：Objective To investigate the expression of epidermal growth factor receptor（EGFR） protein and gene amplification in oral squamous cell carcinoma（OSCC） and evaluate the value of EGFR in occurrence，development and prognosis of OSCC. Methods Immunohistochemical SP method and in situ chemical hybridization（FISH）were used to detect the expression of EGFR protein，gene amplification in 103 cases of OSCC and 30 cases of normal oral mucosa in paraffin-embedded tissue chip material. Results The positive rate of EGFR expresion in OSCC and normal oral mucosa tissues were 42.7% and 3.3%，respectively；the positive rate of EGFR gene amplification was 31.1% and 0，respectively. There were significant differences between OSCC and oral mucosa tissues in the expression and amplification of EGFR. The expression and amplification of EGFR in OSCC had no correlation with age，gender，size or differentiation degree of tumor（P > 0.05），but were correlated with the lymph node metastasis and clinical stage（P < 0.05）. Conclusion EGFR is closely related to the occurrence and development of OSCC，and can be considered as an important index for determining the biological behavior of OSCC.

Key words: OSCC, EGFR, immunohistochemistry, fluorescence

摘要：

目的 探讨表皮生长因子受体（EGFR）在口腔鳞状细胞癌（OSCC）中的蛋白表达与基因扩增，并评估其在OSCC发生、发展和预后中的价值。方法 以石蜡包埋组织芯片为材料，分别采用免疫组化SP法与荧光原位杂交（FISH）技术，检测辽宁省人民医院2003年1—6月手术切除并经病理确诊为OSCC存档石蜡包埋组织蜡块103例及30例正常口腔黏膜组织中的EGFR蛋白表达及基因扩增。结果 EGFR蛋白在OSCC、正常口腔黏膜组织中表达的阳性率分别为42.7%和3.3%；扩增阳性率分别为31.1%和0；OSCC中EGFR蛋白表达、基因扩增阳性率与正常口腔黏膜组织比较，差异有统计学意义（P < 0.05）；在OSCC组织中EGFR蛋白表达、基因扩增阳性率与患者年龄、性别、肿瘤大小、分化程度等均无相关性（P > 0.05），与肿瘤淋巴结转移、临床分期相关（P < 0.05）。结论 EGFR与OSCC的发生、发展有关，可将其作为判断OSCC生物学行为的重要参考指标。

关键词: 口腔鳞状细胞癌, 表皮生长因子受体, 免疫组织化学, 荧光