Abstract:

Abstract：Objective To investigate expression and distribution of metabotropic glutamate receptor mGluR5 in human dental pulp. Methods Human premolars and third molars（n = 5） that were extracted for orthodontic or other treatment from patients at the Department of Oral Surgery，Shandong University Oral Hospital，were collected. The expression and distribution mGluR5 in human normal pulp were determined by immunohistochemistry. Digital image processing was used to analyse the expression of mGluR5. Results The expression of mGluR5 was positive in the whole normal pulp，while the expression of mGluR5 was significantly weaker from cornal，cervical portion to root of normal pulp in turn. Conclusion The expression and distribution differences of mGluR5 in pulp suggest that it may play an important role in transduction of pulp nociceptive signals.

Key words: metabotropic glutamate receptors 5（mGluR5）, pulp, odontoblast, immunohistochemistry

摘要：

目的 研究代谢性谷氨酸受体第5亚型（mGluR5）在牙髓各部位中的表达及分布。 方法 收集2009年7月至2010年1月山东大学口腔医院口腔颌面外科因正畸或其他治疗需要而拔除的健康前磨牙或第三磨牙5例，制成一系列石蜡切片，利用免疫组化方法检测牙髓各部位mGluR5的表达及分布情况，利用图像分析系统对其表达强度进行半定量分析，探讨mGluR5在牙髓中的作用和意义。结果 正常牙髓从冠部、颈部到根部牙髓成牙本质细胞中mGluR5表达均呈阳性，且由冠部、颈部到根部mGluR5表达强度依次降低。结论 mGluR5在牙髓疼痛传递过程中可能具有一定的作用。

关键词: 代谢性谷氨酸受体第5亚型, 牙髓, 成牙本质细胞, 免疫组化