Abstract:

Objective    To investigate the expression of p16 protein in the tissues of Maxillary Sinus Squamous Cell Carcinoma （MSSCC） and analyze its relationship with the clinical pathological characteristics. Methods    Immunohistochemistry （IHC） was used to detect the expression of p16 protein in 34 cases of maxillary sinus squamous cell carcinoma and 10 cases of normal nasal mucosa. Results    Positive expression rates of p16 protein in maxillary sinus squamous cell carcinoma and normal nasal mucosa tissue were 32.35% and 100.0%，which in the poor differentiation group was significantly lower than that in the good-differentiation group （P < 0.05）. Conclusion    The expression of p16 protein is significantly lower in the maxillary sinus squamous cell carcinoma and correlates with the degree of differentiation. The higher the degree of malignancy，the lower the expression. The results suggest that p16 protein may play a role in the occurrence and development of maxillary sinus squamous cell carcinoma.

Key words: p16；maxillary sinus neoplasms；carcinoma, squamous cell；immunohistochemistry

摘要：

目的    探讨p16蛋白在上颌窦鳞状细胞癌 （maxillary sinus squamous cell carcinoma，MSSCC） 组织中的表达，分析其与MSSCC临床病理特征的关系。方法    收集2010年10月至2015年6月于中国医科大学附属第一医院耳鼻咽喉科手术切除或活检并经病理证实的MSSCC蜡块标本34例（MSSCC组），另取鼻中隔偏曲矫正术中正常下鼻甲黏膜组织10例（正常鼻黏膜组），采用免疫组化SP法分别检测MSSCC和正常鼻黏膜组织中p16蛋白的表达情况。结果    MSSCC组和正常鼻黏膜组中的p16蛋白阳性表达率分别为32.35%（11/34）和100.0%（10/10），差异有统计学意义（P < 0.05），且低分化MSSCC组p16蛋白阳性表达率明显低于高中分化组（P < 0.05）。结论    p16蛋白在MSSCC组织中明显低表达，且表达与肿瘤的分化程度有关，恶性程度越高，其表达越低，提示p16蛋白可能与MSSCC的发生、发展有关。

关键词: p16, 上颌窦肿瘤, 癌, 鳞状细胞, 免疫组织化学