Expression of PFP and GrB in gastric cancer and their clinical significance        HE Chun-hua. Department of General Surgery，Affiliated Hospital of Luzhou  Medical College，Luzhou 646000, China
Abstract    Objective    To study express and clinical significance of PFP and GrB in patient with gastric cancer. Methods    The expression of PFP and GrB in stomach incision tissue, peripheral tissue of gastric cancer, cancer tissue and corresponding tissues of lymphoid metastases was detected by immunohistochemical PV staining between 2008 and 2009 in the Department of General Surgery of Affiliated Hospital of Luzhou Medical College. The expression was analyzed combined with clinical and pathological features. Results    ⑴Comparing the expression of GrB（+） in stomach incision tissue, peripheral tissue of gastric cancer and cancer tissue, there is no significant difference in the adjacent difference two groups (P> 0.05). ⑵Comparing the expression of GrB in corresponding stomach incision tissue and peripheral tissue of gastric cancer for the the same patient with GrB（+） and GrB（-） , there was statistically significant difference (P <0.01). ⑶Taking the PFP cells as positive control, it showed that the PFP protein showed no expression in the stomach incision tissue, peripheral tissue of gastric cancer and the cytoplasm of adjacent epithelial cells of gastric cancer tissues, whether the GrB（+） expressed or not. ⑷Comparing the expression of GrB in gastric cancer and lymph node metastasis, there is no significant difference (P= 0.130). The PFP expressed negative in all lymph node metastasis. ⑸Analyzing the GrB expression in gastric cancer and the corresponding clinical and pathological features, it showed that the expression of GrB in primary tumor may play an inhibitory effect on lymph node metastasis. Conclusion Non-co-expression between GrB（+） gastric cancer cells and PFP has not demonstrated that the GrB（+） gastric cancer cells have lymphoid cells phenotype. GrB may be involved in the whole process of gastric cancer, which is an early event in the formation of gastric cancer.