Clinical research of high risk human papillomavirus infection in breast cancer WANG Chong-jie*, GAO Hai-dong, WANG Lei-yi, et al. *Department of Breast Surgery, Qi Lu Hospital of Shandong University, Jinan 250012, China Corresponding author: MA Rong, E-mail: malone@sdu.edu.cn Abstract Objective To examine the infection of high risk human papillomavirus (HR-HPV) in breast cancer and determine the association between HR-HPV infection and clinical and pathological characters of breast cancer. Methods The exfoliated cells were collected from 85 malignant and 20 benign breast tumors. 13 subtypes HR-HPV DNA were detected by hybrid capture 2(HC2) system. ER, PR and Her-2 of all the breast tumors were also detected. Results HR-HPV DNA was positive in 26 out of 85 breast cancer tumors and none of 20 benign breast diseases(P =0.01). 69.2% breast cancer tumors was HR-HPV DNA positive before age 40 while in the patients after age 40 the HR-HPV DNA positive rate was 23.6% (P =0.027). There was no difference between the breast tumors with different clinical and pathology characters. Conclusions HR-HPV DNA infection exists in breast cancer tissues and it probably runs a roll in the onset of breast cancer.