Comparative study on gut barrier dysfunction and bacterial translocation in patients following hepatolobectomy using half liver vascular occlusion with total liver vascular occlusionQIAO Zhi*,LI Rong,LI Zhanliang,et al.*Department of General Surgery,General Hospital of PLA,Beijing 100853,China Corresponding author:QIAO Zhi,Email:drqiaozhi@yahoo.com.cn AbstractObjectiveTo investigate gut barrier dysfunction and bacterial translocation (BT) after hepatectomy using half or total liver vascular occlusion.MethodsFiftyfive patients undergoing selective hepatectomy between 2003 and 2006 at General Hospital of PLA were divided into two groups:half liver occlusion group (HLO) and total liver occlusion group (TLO).Blood samples were collected prior to and 2h,24h,48h after surgery for microbial DNA extraction,plasma Dlactate,LPS measurement.PCR was performed after DNA extraction with target 16S rRNA gene of most pathogenic bacteria.ResultsNo bacterial DNA was detected before surgery, but it was positive in 32 postoperative patients.The PCRpositive rate, plasma levels of Dlactate and endotoxin of TLO were higher than that of HLO (P<001).ConclusionHalf liver vascular occlusion has the advantages of both decreasing gut barrier injury and bacterial translocation rate.