Objective:To study application of threedimensional reconstruction and visual simulation surgery on pancreas based on the data of 64-row helical computed tomograph (CT) scanning. Methods:To collect the data of pancreatopaphy on 64-row helical CT scanning,the automatic extraction and segment were carried on to the CT sequence images based on the algorithm of auto adapted region growing.The images which had been segmented were proceeded the 3D reconstruction by medical image proceeding system which was designed by us.Then reconstructed objectives were imported into FreeForm Modeling System in the form of STL format to embellish and smooth.Using the GHOST SDK software of FreeForm Modeling System Various instruments were developed.Investigate the visual simulation surgery of pancreas with PHANTOM software. Results:It is rapid and effective that the image sequence segmentation of pancreas is applied by the algorithm of auto adapted region growing.The 3D reconstruction result of pancreas by medical image processing software show clearly and has vivid appearance.The visual simulation surgery of pancreas is high simulated,vivid,exact and give the feeling of ‘strength’ and make people feel operating a real surgery on an operation table. Conclusion:3D reconstruction and simulation surgery based on helical CT scanning has important application value on the operation planning selection,risk assessment and clinical training.