Objective:To analyze variations of main trunk of portal vein (MTPV) in healthy persons by multislice spiral computed tomography portagraphy (MSCTP). Methods:Four hundreds and five subjects （including 200 normal volunteers）were performed by test bolus to demonstrate the optimization of MSCTP from December 2006 to June 2007 at the Affiliated Zhujiang Hospital of South Medical University.The maximum intensity prejection (MIP),volume rendering (VR) and multiplanar reformation (MPR) were employed for threedimensional reconstruction to analyze variations of MTPV (1～3 grades). Results:There were 12 types of MTPV (1～3 details) with the variation rate of 2074%. Conclusion:Due to high variation rate of portal vein among normal people,the 3-D reconstruction of multislice CT is constructive to local the focus and the decision of approaches for the surgical treatment in clinic.