Objective:Observe the effects of the anal fistula treatment with acellular dermal matrix. Methods:46 case severy kind of anal fistula patients were randomly divided into 2 groups:treatment group (23 cases) Were treated with acellular extracellular matrix.Control group (23 cases) were treated with operations of anal fistula amputate. Results:The ache、healing time and anal deformation of treatment group were obviously superior to that of control group.The healing rate of anal fistula for treatment group is 100%. Conclusion:This method has advantages of mininal invasion,light pain,shorter course,protecting anal function and formation.