Objective:To construct the lentiviral expression vector of HLA-E gene and investigate its significance for further study on tumor immunity. Methods:After the lentiviral expression vector of HLA-E gene (pGC-E1-GFP-HLA-E) was transduced HepG2 cell lines,the transduction efficiency，the HLA-E mRNA level by RT-PCR and the expression of HLA-E by immunohistochemistry were detected between October 2006 and November 2007 in the Liver Transplautation Center of Third Affiliated Hospital of Sun Yat-sen University. Results:The transduction efficiency of HepG2 cell lines using the lentiviral vectors was 95.0%±1.3% which allowing the stable expression of HLA-E after 72 hr.The HLA-E-mRNA level in vitro was detected more 8.73±1.05 times and 293.48±42.01 times than normal level by RT-PCR (P＜0.01).The expression of HLA-E by immunohistochemistry was displayed significant enhancement. Conclusion:The lentiviral expression vector is ideal vetor of gene therapy and made genes transducted stable expression in target cell.