Objective:To summarize the experience of diagnosis of solid pseudopapillary tumor of pancreas (SPT). Methods:Clinical manifestations, laboratory examination, imageology, pathology of 24 patients with SPT admitted in general hospital of the People’ Liberation Army from February 2000 to March 2007 were retrospectively analyzed. Results:23 of 24 patients were female and the mean age was 244 years. Clinical presentation included a palpable abdominal mass in 5 patients, abdominal pain in 7 cases,diarrhea in 1 patient, nausea and abdominal distention in 2 cases.9 patients were asymptomatic.Tumor markers in all patients were negative.Abdominal imaging showed solid and solid cystic masses in pancreas or abdominal cavity.13 cases located in the head of pancreas,4 in the body of pancreas,7 in the tail of pancreas.2 cases received preoperative ultrasonic guidance biopsy and 6 underwent freezing pathology around surgery,1 case and 3 cases were diagnosed as SPT respectively. Conclusion:The correct diagnosis of SPT depends on comprehensive analysis of clinical feature,image and histopathologic characteristic.