目的    寻找筛查大肠癌早期肝转移病人血清特异性蛋白。方法    对中国人民解放军第202医院2007-2009年收治的20例大肠癌和20例大肠癌早期肝转移病人，应用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）技术检测其血清中特异性蛋白的相对含量。结果    两组样本质荷比在2000～30 000u间有3774u、11 851u的2个蛋白峰有显著差异。结论    SELDI-TOF-MS技术对大肠癌早期肝转移的诊断和特异性肿瘤标记物的筛选等方面具有一定价值，值得推广。

Study of searching for the serum proteomic patterns of early liver metastasis of colorectal cancer by SELDI- TOF-MS        CAI Xiang-jun*，WANG Fu-rong, WANG Chun-xi, et al. *Department of General Surgery, 202 Hospital of PLA，Shenyang110003,China
Corresponding author: CAI Xiang-jun, E-mail：caixiangjun61@126.com
Abstract     Objective     To detect the serum proteomic patterns in early liver metastasis of colorectal cancer patients by surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI- TOF-MS). Methods     The relative contents of serum proteins of 20 colorectal cancer patients and 20 early liver metastasis of colorectal cancer patients were detected by WCX2 chip and SELDI-TOF-MS between 2007 and 2009 in 202 Hospital of PLA. Results     At the M/Z values range from 2000u to 30 000u, the contents of two proteins (3774u and 11 851u) had significant difference in the two groups. Conclusions     SELDI-TOF-MS shows great potentiality for early liver metastasis of colorectal cancer in diagnosis and screening of tumor biomarkers, and deserves generalization.