Abstract:

Abstract： Objective To research into the expression of GPR30 mRNA and protein in myometrial tissue before and after the onset of labor and their relativity with parturition. Methods Between March 2011 and March 2012, full-term pregnancy women were selected in Qingdao Municipal Hospital. Those who chose elective cesarean section at term were grouped as nonlaboring (n=40) and others who required emergency cesarean section after the active period of labor were categorized as laboring (n=50). Then, a small amount of myometrial tissue strips were collected respectively from two groups during cesarean section and tested by Real-time PCR and Western blot for the expression of GPR30 mRNA and protein. And venous blood was also extracted before operation to verify the amount of E2, E3 and P via radioimmunoassay.Results Real-time PCR showed GPR30 mRNA expressed in myometrial tissue strips of both groups. Means of △Ct for the laboring group and the nonlaboring group were 3.08±0.35 and 4.02±0.67 correspondingly, which displayed statistical significance (P<0.05) in their difference. Relative quantitative analysis made by Double △Ct revealed that the expression of GPR30 mRNA for the laboring was 1.92 times more than that of the nonlaboring. Obviously, the laboring group had higher level of serum E2 and E3 [（7882.41±921.76）pmol/L，（544.12±15.58）nmol/L] than the other group [（5210.72±873.13）pmol/L，（326.36±12.26）nmol/L].Using Western blot, we detected GPR30 protein in myometrial tissue strips of two groups. Its level in the laboring group (4.06±0.25) was apparently higher than that of the nonlaboring group (1.94±0.23), and the difference showed statistical significance (P<0.05). Conclusion The high expression of GPR30 may participate in the regulation of estrogen to uterine contraction during pregnancy and parturition.

Key words: G protein-coupled receptor 30, myometrium, estrogen receptor, estrogen, parturition

摘要：

目的 探讨G蛋白偶联受体30（GPR30） mRNA和蛋白在临产前、后子宫平滑肌组织中的表达及其与分娩发动的相关性。方法 2011年3月至2012年3月在青岛市市立医院选取临产前进行选择性剖宫产的足月妊娠妇女作为未临产组（n=40），自然临产进入产程活跃期后进行急症剖宫产的足月妊娠妇女作为临产组（n=50），于剖宫产术中取少量子宫平滑肌组织，采用实时荧光定量PCR技术和Western blot技术检测两组子宫平滑肌组织中GPR30 mRNA和蛋白的表达量；并于术前取静脉血，采用放射免疫法测定血清中雌二醇（E2）、雌三醇（E3）以及孕酮（P）的含量。结果 实时荧光定量PCR方法检测临产组和未临产组子宫平滑肌组织中均有GPR30 mRNA表达，临产组样本△Ct为3.08±0.35，未临产组样本△Ct为4.02±0.67，差异具有统计学意义（P＜0.05），用双△Ct法进行相对定量分析，临产组GPR30 mRNA表达量是未临产组的1.92倍。Western blot技术检测临产组和未临产组子宫平滑肌组织中均有GPR30蛋白表达，临产组GPR30蛋白相对表达量4.06±0.25明显高于未临产组1.94±0.23，差异有统计学意义(P＜0.05)。临产组血清E2、E3浓度[（7882.41±921.76）pmol/L，（544.12±15.58）nmol/L]均显著高于未临产组[（5210.72±873.13）pmol/L，（326.36±12.26）nmol/L]。结论 足月妊娠分娩发动时GPR30高表达可能参与介导雌激素对子宫收缩的调节作用。

关键词: GPR30, 子宫平滑肌, 雌激素受体, 雌激素, 分娩发动