Specific expression and significance of endocrine gland-derived vascular endothelial growth factor gene in ectopic and eutopic endometrium of patients with endometriosis.

doi:10.7504/fk2015030115

Abstract

Abstract:

Abstract： Objective To investigate the expression and significance of endocrine specific vascular endothelial growth factor (EG-VEGF) in endometriosis and its lateral tissue.Methods Detected the expression of EG-VEGF by immunohistochemistry in 32 cases of endometriosis, eutopic and ectopic endometrium, the lateral tissue of endometriosis, control group 1 (32 case of normal endometrium) and control group 2 (15 cases of normal ovaries).Results The expression and positive expression rate of EG-VEGF in ectopic endometrium were higher than eutopic endometrium[1.99±0.26(84.3%),1.65±0.34(65.6%),respectively](P<0.05 and P<0.01);the expression and positive expression rate of EG-VEGF in eutopic endometrium were higher than control group1[1.65±0.34 (65.6%),0.85±0.29(21.9%),respectively](P<0.05);the expression and positive expression rates of EG-VEGF in ectopic endometrium was higher than ovarian of ectopic lesion side[1.99±0.26(84.3%),[1.45±0.28(56.2%),respectively](P<0.05);the expression and positive expression rates of EG-VEGF in ovarian of ectopic lesion side were higher than control ovarian[1.45±0.28(56.2%), 0.89±0.31(40.0%)respectively](P<0.01 and P<0.05); between the two groups, the differences were highly statistically significant.Conclusion The abnormally high level of EG-VEGF in endometriosis lesions, its side tissue and eutopic endometrium is associated with the occurrence and development of endometriosis.

Key words: endometriosis, endocrine gland-derived vascular endothelial growth factor, angiogenesis

摘要：

目的探讨内分泌腺特异性血管内皮生长因子(EG-VEGF)在子宫内膜异位症（内异症）组织及异位病灶旁组织的表达及意义。方法采用免疫组化法检测2013年8月至2014年8月在中国医科大学附属盛京医院住院的32例内异症患者在位内膜、异位内膜、异位病灶旁卵巢及32例正常子宫内膜（对照组1）、15例正常卵巢（对照组2）中EG-VEGF的表达。结果异位内膜EG-VEGF表达量及阳性表达率[1.99±0.26(84.3%)]，均高于在位内膜[1.65±0.34(65.6%）]，P<0.05及P<0.01；在位内膜EG-VEGF表达量及阳性表达率[1.65±0.34 (65.6%)]，均高于对照组内膜[0.85±0.29(21.9%)]，P<0.05。异位内膜EG-VEGF表达量及阳性表达率[1.99±0.26(84.3%)]，均高于异位病灶旁卵巢组织[1.45±0.28(56.2%)]，P<0.05；异位病灶旁卵巢EG-VEGF表达量及阳性表达率[1.45±0.28(56.2%)]均高于对照组卵巢[0.89±0.31(40.0%)]，P<0.01及P<0.05。组间比较，差异均有统计学意义。结论子宫内膜异位症患者在位内膜、异位内膜及异位病灶旁组织中EG-VEGF表达的异常升高与内异症的发生及发展相关。

关键词: 子宫内膜异位症, 内分泌腺特异性血管内皮生长因子, 血管生成

CLC Number:

R711.71

MA Yue,LI Yan,LIU Kui-ran，WANG Dan-bo.. Specific expression and significance of endocrine gland-derived vascular endothelial growth factor gene in ectopic and eutopic endometrium of patients with endometriosis.[J]. Acta Metallurgica Sinica, DOI: 10.7504/fk2015030115.

马悦，李妍，刘岿然，王丹波. 子宫内膜异位症异位及在位内膜EG-VEGF基因表达及意义[J]. 中国实用妇科与产科杂志, DOI: 10.7504/fk2015030115.

References

［1］ Bulun SE. Endometriosis［J］. N Engl J Med, 2009, 360(3): 268-279.

［2］ Grund EM, Kagan D, Tran CA, et al. Tumor necrosis factor-alpha regulates inflammatory and mesenchymal responses via mitogen-activated protein kinase kinase, p38, and nuclear factor kappaB in human endometriotic epithelial cells［J］. Mol Pharmacol, 2008, 73(5): 1394-1404.

［3］ Rocha AL, Reis FM, Taylor RN. Angiogenesis and endometriosis［J］. Obstet Gynecol Int, 2013: 859619.

［4］ Brouillet S, Hoffmann P, Feige JJ, et al. EG-VEGF: a key endocrine factor in placental development［J］. Trends Endocrinol Metab,2012, 23(10): 501-508.

［5］ Almog B, Shehata F, Sheizaf B, et al. Effects of ovarian endometrioma on the number of oocytes retrieved for in vitro fertilization［J］. Fertil Steril,2011, 95(2): 525-527.

［6］ Goteri G, Lucarini G, Montik N, et al. Expression of vascular endothelial growth factor (VEGF), hypoxia inducible factor-1alpha (HIF-1alpha), and microvessel density in endometrial tissue in women with adenomyosis［J］. Int J Gynecol Pathol, 2009, 28(2): 157-163.

［7］ LeCouter J, Kowalski J, Foster J, et al. Identification of an angiogenic mitogen selective for endocrine gland endothelium［J］. Nature, 2001, 12(6850): 877-884.

［8］ Brouillet S, Hoffmann P, Chauvet S, et al. Revisiting the role of hCG: new regulation of the angiogenic factor EG-VEGF and its receptors［J］. Cellular Mol Life Sci:CMLS, 2012, 69(9): 1537-1550.

［9］   Balu S, Pirtea L, Gaje P, et al. The immunohistochemical expression of endocrine gland-derived-VEGF (EG-VEGF) as a prognostic marker in ovarian cancer［J］. Rom J Morphol Embryol,2012,53(3): 479-483.

［10］    Ferrara N, Frantz G, LeCouter J, et al. Differential expression of the angiogenic factor genes vascular endothelial growth factor (VEGF) and endocrine gland-derived VEGF in normal and polycystic human ovaries［J］.American J Pathol,2003,162(6): 1881-1893.

［11］   Battersby S, Critchley HO, Morgan K, et al. Expression and regulation of the prokineticins (endocrine gland-derived vascular endothelial growth factor and Bv8) and their receptors in the human endometrium across the menstrual cycle［J］.J Clin Endocrinol Metab,2004,89(5):2463-2469.

［12］    Ngan ES, Lee KY, Yeung WS, et al. Endocrine gland-derived vascular endothelial growth factor is expressed in human peri-implantation endometrium, but not in endometrial carcinoma［J］. Endocrinology, 2006, 147(1): 88-95.

（2014-12-15 收稿 2015-01-07 修回）

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