Abstract:

Objective    To study the cytotoxic effects of OK-432 on the two human oral squamous cell carcinoma Tca8113 and BcaCD885 cell lines. Methods    The inhibitory effects and the 50% inhibition concentration values（IC50）of OK-432 against Tca8113 and BcaCD885 with different drug concentration were evaluated by methyl thiazolyl tetrazolium（MTT）. The time-dependent cytotoxic effects of OK-432 were in 1-5 days，and the dose-effect relationship was investigated at the 4th day. Results    OK-432 had high anticancer effects on Tca8113 and BcaCD885，and compared with the control group they were significantly higher（P < 0.05），and the cytotoxic effects were dose-dependent. Conclusion        OK-432 significantly inhibits the proliferation of  Tca8113 and BcaCD885 cell，and the inhibition on Tca8113 is stronger than on BcaCD885.

Key words: OK-432, oral squamous cell carcinoma, cell proliferation

摘要：

目的    探讨OK-432对人口腔鳞癌细胞系Tca8113和BcaCD885的药物敏感性。方法    采用四基偶氮唑蓝（MTT）法检测不同剂量OK-432在5个时间点（1、2、3、4、5 d）对Tca8113和BcaCD885的生长抑制作用，计算不同时间点OK-432的半数抑癌率（IC50）和生长抑制率，选择药物作用后第4天观察量效关系。结果    OK-432 能明显抑制Tca8113和BcaCD885细胞的增殖生长，与对照组比较差异有统计学意义（P＜0.05），其抑制效应随药物浓度升高而增高。结论    OK-432能显著抑制Tca8113和BcaCD885增殖，对Tca8113的敏感性比BcaCD885强。

关键词: OK-432, 口腔鳞癌系, 敏感性