Abstract: Objective　To analyze the correlation between FeNO values of different levels in children with bronchial asthma and IL-9，IL-17 in peripheral blood. Methods　A total of 80 children diagnosed with asthma，who were admitted to the Outpatient Department of Children’s Hospital of Soochow University from October 2017 to June 2019，and 20 normal control children from the physical examination center were randomly selected. According to the FeNO value of 25×10-9，children with asthma were divided into FeNO≥25×10-9 group（FeNO high group） and FeNO＜25×10-9 group（FeNO low group）. The levels of IL-4，IL-5，IL-9 and IL-17 in the serum of each group were detected by ELISA kit，and the lung function indexes were detected in each group，including FEV1/FVC%，MEF25，MEF50 and MEF75. The correlation between FeNO levels of children with asthma and IL-4，IL-5，IL-9，IL-17 as well as lung function indexes in peripheral blood was analyzed. Results 　The levels of IL-4，IL-5，IL-9 and IL-17 in peripheral blood of children with FeNO≥25×10-9 were higher than those of FeNO＜25×10-9 group and control group，while those of FeNO＜25×10-9 group were also higher than those of the control group，the differences being statistically significant（P＜0.05）. The lung function indexes of children in FeNO≥25×10-9 group and those in FeNO＜25×10-9 group were lower than those in the control group（P＜0.05）. There was no difference in lung function indexes between children with FeNO≥25×10-9 and those with FeNO＜25×10-9（P＞0.05）. Pearson correlation analysis showed that FeNO level was positively correlated with IL-4，IL-5，IL-9 and IL-17 in peripheral blood（P＜0.05），and had no correlation with lung function indexes（FEV1/FVC%，MEF25，MEF50 and MEF75）（P＞0.05）. Conclusion　The level of FeNO is positively correlated with IL-9 and IL-17 in peripheral blood. FeNO and IL-9，IL-17 together with other cytokinescan jointly reflect airway inflammation，and can reflect the children’s condition with asthma more sensitively than lung function indexes.

Key words: asthma, FeNO, cytokines, pulmonary function, child

摘要： 目的　分析支气管哮喘患儿呼出气一氧化氮（FeNO）值在不同水平下与外周血中白介素（IL）-9、IL-17的相关性。 方法　随机选取2017年10月至2019年6月苏州大学附属儿童医院门诊就诊的80例哮喘患儿及20例体检中心来源的正常对照组儿童，以FeNO值25×10-9为界限值将哮喘患儿分为FeNO≥25×10-9组（FeNO high组）和FeNO＜25×10-9组（FeNO low组）。用酶联免疫吸附（ELISA）试剂盒检测各组血清中IL-4、IL-5、IL-9、IL-17的水平，同时每组均行肺功能指标检测，包括第1秒用力呼气量占用力肺活量比值（FEV1/FVC%）、用力呼气25%容量（MEF25）、MEF50、MEF75。分析哮喘儿童FeNO水平与外周血中IL-4、IL-5、IL-9、IL-17及肺功能指标的相关性。结果　FeNO high组外周血中IL-4、IL-5、IL-9、IL-17水平均高于FeNO low组和对照组，而FeNO low组也均高于对照组，差异均有统计学意义（P值均＜0.05）。FeNO high组和FeNO low组肺功能指标均低于对照组儿童，差异有统计学意义（P值均＜0.05），但FeNO high组与FeNO low组肺功能指标比较差异均无统计学意义（P＞0.05）。Pearson相关分析显示FeNO水平与外周血中IL-4、IL-5、IL-9、IL-17呈正相关（P值均＜0.05），与肺功能指标FEV1/FVC%、MEF25、MEF50、MEF75无相关性（P值均＞0.05）。 结论　在哮喘的非急性发作期FeNO水平与外周血中IL-9、IL-17呈正相关，IL-9、IL-17等中性粒细胞相关细胞因子也共同反映气道炎症，并较肺功能更能敏感地反映哮喘患儿病情。

关键词: 哮喘, 呼出气一氧化氮, 细胞因子, 肺功能, 儿童