Comparison of miRNAs expression profiles between human dental pulp stem cells and non-stem cells.

Acta Metallurgica Sinica

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Comparison of miRNAs expression profiles between human dental pulp stem cells and non-stem cells.

LV Hong-bing*，ZHENG Bi-qiong，LEI Li-shan，ZHANG Ming，FU Sheng， FAN Li-na

*The Affiliated Stomatological Hospital of Fujian Medical University， Fuzhou 350002，China

Online:2014-03-15 Published:2014-03-31

人牙髓干细胞及非牙髓干细胞中微小RNA表达谱比较研究

吕红兵1，郑碧琼1，雷丽珊1，张明1，傅升2，樊丽娜2

作者单位：1.福建医科大学附属口腔医院牙体牙髓一科，福州350002；2.南京军区福州总医院口腔科，福州350002

通讯作者: 吕红兵
基金资助:
福建省自然科学基金面上项目（2011J01169）；福建省科技厅重点项目（ 2011Y0024）

Abstract

Abstract:

Abstract： Objective To observe the expression of miRNAs between human dental pulp stem cells （DPSCs） and human dental pulp non-stem cells and to analyze the roles of miRNA in the stemness of DPSCs. Methods DPSCs were isolated by immune-magnetic beads binding specific STRO-1 antibody separation system. Mineralization medium-induced odontoblasts-like cells differentiated from DPSCs were tested by ALP， OC values and von Kossa staining. Differential miRNAs of human dental pulp stem cells and non-stem cells were screened by microRNA array. Results miRNA microarrays revealed that 11 miRNAs were upregulated in DPSCs while 3 miRNAs were downregulated compared with non-stem cells. Conclusion Stemness of DPSCs may have a close relationship with differential miRNAs expression.

Key words: dental pulp stem cells, miRNAs, profile, stemness

摘要：

目的比较微小RNA（miRNAs）在人牙髓干细胞（dental pulp stem cells， DPSCs）及非DPSCs中的表达差异，探讨其在维持DPSCs干性状态中的作用。方法本研究于2013年1—10月在福建医科大学附属口腔医院完成。原代培养人牙髓细胞，利用结合了STRO-1特异性抗体的免疫磁珠分选获得DPSCs，并进行成牙本质样细胞的诱导分化，检测碱性磷酸酶（ALP）、骨钙素（OC）值以及进行von Kossa染色，鉴定其分化能力。采用miRNA基因芯片技术，检测DPSCs和非DPSCs中miRNAs的表达，筛选出差异表达的miRNAs。结果与非DPSCs相比，DPSCs中表达上调超过2倍的miRNAs有11个，表达下调超过2倍的miRNAs有3个。结论 miRNAs表达谱的变化可能与DPSCs干性状态的维持相关。

关键词: 牙髓干细胞, miRNAs, 表达谱, 干性

CLC Number:

Ｒ78

LV Hong-bing*，ZHENG Bi-qiong，LEI Li-shan，ZHANG Ming，FU Sheng， FAN Li-na. Comparison of miRNAs expression profiles between human dental pulp stem cells and non-stem cells.[J]. Acta Metallurgica Sinica.

吕红兵，郑碧琼，雷丽珊，等. 人牙髓干细胞及非牙髓干细胞中微小RNA表达谱比较研究[J]. 中国实用口腔科杂志.

References

［1］ Gronthos S， Mankani M， Brahim J，et al. Postnatal human dental pulp stem cells （DPSCs） in vitro and in vivo［J］. Proc Natl Acad Sci USA， 2000， 97（25）：13625-13630.

［2］ Lagos-Quintana M， Rauhut R， Lendeckel W， et al. Identification of novel genes coding for small expressed RNAs［J］. Science，2001，294（5543）：853-858.

［3］ Dennis JE， Carbillet JP， Caplan AI， et al. The STRO-1+ marrow cell population is multipotential［J］. Cells Tissues Organs， 2002， 170（2-3）：73-82.

［4］ Ahdjoudj S， Lasmoles F， Oyajobi BO， et al. Reciprocal control of osteoblast/chondroblast and osteoblast/adipocyte differentiation of multipotential clonal human marrow stromal F/STRO-1（+） cells［J］. J Cell Biochem ，2001， 81（1）：23-38.

［5］ Yang X， Zhang W， van den Dolder J， et al. Multilineage potential of STRO-1+ rat dental pulp cells in vitro［J］. J Tissue Eng Regen Med， 2007， 1（2）：128-135.

［6］ Yang X， van den Dolder J， Walboomers XF， et al. The odontogenic potential of STRO-1 sorted rat dental pulp stem cells in vitro［J］. J Tissue Eng Regen Med， 2007， 1（1）：66-73.

［7］ Song L， Tuan RS. MicroRNAs and cell differentiation in mammalian development［J］. Birth Defects Res C Embryo Today， 2006， 78（2）：140-149.

［8］ Alvarez-Garcia I， Miska EA. MicroRNA functions in animal development and human disease［J］. Development，2005， 132（21）：4653-4662.

［9］ Mizuno Y， Yagi K， Tokuzawa Y， et al. miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation［J］. Biochem Biophys Res Commun，2008， 368（2）：267-272.

［10］ Luzi E， Marini F， Sala SC， et al. Osteogenic differentiation of human adipose tissue-derived stem cells is modulated by the miR-26a targeting of the SMAD1 transcription factor［J］. J Bone Miner Res， 2008， 23（2）：287-295.

［11］ Yi R， Poy MN， Stoffel M， et al. A skin microRNA promotes differentiation by repressing 'stemness'［J］. Nature， 2008， 452（7184）：225-229.

［12］ Han J， Ito Y， Yeo JY， et al. Cranial neural crest-derived mesenchymal proliferation is regulated by Msx1-mediated p19（INK4d） expression during odontogenesis［J］. Dev Biol，2003， 261（1）：183-196.

［13］ Yu KR， Lee S， Jung JW， et al. MicroRNA-141-3p plays a role in human mesenchymal stem cell aging by directly targeting ZMPSTE24［J］. J Cell Sci， 2013，126（Pt23）：5422-5431.

［14］ Lee MR， Prasain N， Chae HD， et al. Epigenetic regulation of NANOG by miR-302 cluster-MBD2 completes induced pluripotent stem cell reprogramming［J］. Stem Cells ，2013， 31（4）：666-681.

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[2]	. [J]. Acta Metallurgica Sinica, 2021, 14(3): 356-359.
[3]	. [J]. Acta Metallurgica Sinica, 2021, 14(3): 347-350.
[4]	. [J]. Acta Metallurgica Sinica, 2021, 14(2): 196-198.
[5]	. [J]. Acta Metallurgica Sinica, 2021, 14(1): 74-80.
[6]	. [J]. Acta Metallurgica Sinica, 2020, 13(7): 418-422.
[7]	. [J]. Acta Metallurgica Sinica, 2020, 13(5): 301-304.
[8]	. [J]. Acta Metallurgica Sinica, 2019, 12(5): 279-284.
[9]	LV Hong-bing*，LU Zhao-jie，ZHENG Bi-qiong，LEI Li-shan， LU Guo-ying， FU Sheng，FAN Li-na. Target gene and gene function of differential expression of microRNA in human periodontal ligament stem cells and non-stem cells. [J]. Acta Metallurgica Sinica, 2014, 7(7): 403-406.
[10]	, , . [J]. , 2011, 4(2): 91-93.
[11]	, . [J]. , 2011, 4(04): 238-241.

Comparison of miRNAs expression profiles between human dental pulp stem cells and non-stem cells.

人牙髓干细胞及非牙髓干细胞中微小RNA表达谱比较研究

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