Abstract:

Abstract： Objective To study the effects of gossypol acetic acid （GAA） on proliferation and the mRNA expressions of E-cadherin in human adenoid cystic carcinoma ACC-M Cell in vitro. Methods （1）MTT assay was used to detect the effects on proliferation in ACC-M cells treated by different concentration of GAA. （2）Real-time fluorescence quantitative PCR（RFQ-PCR）was used to detect mRNA expression level changes of the E-cadherin gene in ACC-M cells treated by GAA for 48h and 72h. Results （1） Experimental GAA group could be observed on growth and proliferation inhibition of E-cadherin cells after being treated by GAA. （2）Compared with the control group， the result of RFQ-PCR showed that mRNA expression of E-cadherin gene in ACC-M cells was increased after GAA treatment （19 μmol/L GAA for 48 h and 10 μmol/L for 72 h）（P < 0.05）. Conclusion GAA can suppress proliferation of ACC-M cells and also increase E-cadherin gene mRNA expression.

Key words: human adenoid cystic carcinoma, gossypol acetic acid, tumor suppressor gene, E-cadherin

摘要：

目的 研究醋酸棉酚（gossypol acetic acid，GAA）对体外培养的人腺样囊性癌ACC-M细胞增殖及E-cadherin基因mRNA表达的影响，初步探讨GAA的抗肿瘤作用机制。方法 本研究于2013年4—7月在昆明医科大学附属口腔医院（云南省高校口腔医学重点实验室）完成。（1）应用四唑盐比色（MTT）法观察GAA对ACC-M细胞生长的作用。（2）应用实时荧光定量（RFQ-PCR）法检测ACC-M细胞在GAA作用48、72 h后E-cadherin 基因mRNA的表达变化。结果 （1）MTT法检测显示：GAA作用ACC-M细胞24、48和72 h后，细胞的生长受到抑制。（2）RFQ-PCR法检测显示：分别以19、10 μmol/L的GAA作用ACC-M细胞48 、72 h 后，细胞中E-cadherin mRNA的表达水平高于对照组（P＜0.05）。结论 GAA能抑制ACC-M细胞生长，增强E-cadherin基因mRNA表达。

关键词: 人腺样囊性癌, 醋酸棉酚, 抑癌基因, E-cadherin