中国实用口腔科杂志

中国实用口腔科杂志

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夏枯草对实验性口腔癌化学预防作用的研究

李 芳1，孙 正2   

  1. 大连大学附属中山医院口腔科
  • 收稿日期:2009-04-02 修回日期:2009-05-10 出版日期:2009-06-15 发布日期:2009-06-15

  • Received:2009-04-02 Revised:2009-05-10 Online:2009-06-15 Published:2009-06-15

摘要：

目的 探讨夏枯草对二甲基苯并蒽（DMBA）诱发的金黄地鼠口腔癌的化学预防作用。方法 2006年3月在首都医科大学口腔医学院研究所将30只金黄地鼠分3组：阴性对照组（6只）不涂药;阳性对照组（12只）涂0.5%DMBA于左侧颊囊，每周3次，共涂3周;夏枯草组（12只）前3周处理同阳性对照组，3周后换涂1%夏枯草，每周3次，涂1周。第4周末实验结束处死所有地鼠，处死前2 h腹腔注射50 mg／kg的5-溴脱氧尿嘧啶（5-bromo-2’-deoxyuridine，BrdU）。取左侧颊囊行组织病理学观察和BrdU免疫组化染色。结果 与阳性对照组相比，夏枯草组颊囊单纯增生和异常增生的病灶数目均有所降低，其单纯增生病灶数目与阳性对照组比较差异有统计学意义（P < 0.05）。夏枯草组炎症细胞数（52.25 ± 18.53）个／mm2与阳性对照组（158.65 ± 26. 51）个／mm2相比，差异有统计学意义（P < 0.01）。夏枯草组 BrdU阳性率与阳性对照组相比有所降低，但二者差异无统计学意义。结论 夏枯草对DMBA诱发的金黄地鼠的炎症和口腔癌前病变的单纯增生有一定的抑制作用，但对于异常增生和细胞增殖抑制作用不明显。

关键词: 夏枯草, 口腔癌前病变, 炎症, 化学预防

Abstract:

Objective To study the preventive effect of Prunella vulgaris L. on 7，12-dimethylbenz（a）anthracene （DMBA）-induced oral precancerous lesions in golden Syrian hamsters. Methods The hamsters were divided into three groups. While the negative control group was not treated，the other Syrian hamsters were applied with 0.5% DMBA solution topically to the left cheek pouch three times per week for three consecutive weeks. After the last treatment of DMBA，the hamsters were divided into two groups at random. The positive group was not treated，another group received Prunella vulgaris L. three times within the following week. The animals were disposed of 4 weeks after the start of the experiment. They were injected with 5-bromo-2’-deoxyuridine （BrdU） 50mg／kg before they were killed. The samples were collected for histopathology and BrdU immunohistochemisty. Results Prunella vulgaris L. significantly decreased the number of inflammatory cells from 158.65 ± 26. 51／mm2 to 52.25 ± 18.53／mm2 （P < 0.01）. The incidence of hyperplasia was decreased from 4.60 ± 1.42 to 1.63 ± 0.65. The incidence of dysplasia was decreased from 3.47 ± 1.12 to 1.82 ± 0.77. Prunella vulgaris L. decreased the frequency of BrdU-index （P > 0.05）. Conclusion The results suggest that Prunella vulgaris L. has some effect on the prevention of DMBA-induced oral precancerous lesions，but the effect is not significant on the suppression of cell proliferation and dysplasia.

Key words: Prunella vulgaris L., oral precancerous lesions, inflammation, chemoprevention