关键词: 组织工程, 口腔黏膜成纤维细胞, 胶原, 壳多糖

Abstract:

Objective    To investigate the feasibility of constructing the lamina propria of oral mucosa with the artificial scaffold of chitosan-collagen gel and oral mucous fibroblasts（OMF）in vitro. Methods    Under sterile condition，2 × DMEM solution，serum of embryo cattle，collagen solution and chitosan were mixed together into gel solution pro rata. Then，the rat OMFs were added into the above gel solution to form the artificial lamina propria of oral mucosa. The cell growth and the gel constriction were observed continuously. Results    The rat germ storage was successfully constructed，and primary and progeny cells were not significantly different in morphology and growth rate. After ten days of in vitro culture of the chitosa-collagen-OMF complex（FPCCL），the OMFs could adhere to artificial scaffold and grow well in the way of the spindle-like and disorder. The OMFs proliferated in the following days，whereas the gel became pale and thin，then constricted after 3 days. Conclusions    The OMFs may grow and secrete substrate in three-dimensional space formed by chitosan-collagen gel，and then form compact connective tissue similar to oral mucous propria. Chitosan-collagen gel is a suitable material for constructing oral mucous propria of tissue engineering.

Key words: tissue engineering；oral mucous fibroblast；collagen；chitosan