中国实用口腔科杂志

中国实用口腔科杂志 ›› 2011, Vol. 4 ›› Issue (06): 345-347.

• 论著 • 上一篇    下一篇

牙周组织工程中壳聚糖-丝素蛋白-磷酸三钙复合物应用研究

  

  1. 山西医科大学 a研究生院，b口腔医院修复科，太原 030001
  • 收稿日期:2011-02-23 修回日期:2011-04-30 出版日期:2011-06-15 发布日期:2011-07-11

  • Received:2011-02-23 Revised:2011-04-30 Online:2011-06-15 Published:2011-07-11

摘要：

目的    探讨以犬牙周膜细胞（periodontal ligament cells，PDLCs）为种子细胞，壳聚糖-丝素蛋白-磷酸三钙复合物作为支架材料，应用于牙周组织工程的优越性。方法    本研究于2010年5月至2011年3月在山西医科大学进行。选择1 ~ 2岁的健康雄性杂种犬6只，以每只犬下颌双侧第2、3、4前磨牙为实验牙，建立雄性杂种犬的牙周缺损模型；模型建立后，应用随机化法将其分为空白对照组、壳聚糖组、壳聚糖-丝素蛋白-磷酸三钙复合物组，每组2只犬。体外培养犬PDLCs，取第4代细胞接种到支架材料上，并于2 h内分别植入犬下颌双侧第2、3、4前磨牙的牙周缺损处；术后8周处死动物，取实验处牙槽骨；经常规固定、脱钙、包埋后，HE染色，光镜下观察牙周组织再生情况。噻唑蓝（MTT）法检测支架材料对犬PDLCs增殖的影响。结果    HE染色结果显示，壳聚糖-丝素蛋白-磷酸三钙复合物支架材料用于牙周组织工程，其牙周间隙减小、成骨细胞数量增多较壳聚糖组和空白对照组更为明显。MTT法显示，壳聚糖-丝素蛋白-磷酸三钙复合物支架对犬PDLCs的生长、增殖影响与单纯壳聚糖作为支架材料相比，差异无统计学意义（P > 0.05）。结论    以犬PDLCs为种子细胞，壳聚糖-丝素蛋白-磷酸三钙复合物支架材料较单纯壳聚糖支架材料具有更强的组织相容性和再生能力，更适合应用于牙周组织工程。

关键词: 牙周膜细胞, 壳聚糖, 丝素蛋白, 磷酸三钙, 组织工程

Abstract:

Objective    To study the advantage of the application of a method to tissue engineering. The method is to take periodontal ligament cells as seed cells and to take chitosan-silk fibroin-tricalcium phosphate as stent meterial. Methods    This study was carried out from May 2010 to March 2011. An experimental model was established in 6 dogs who were about 1 ~ 2 years old；both the premolars were used as the experimental teeth. They were randomly divided into 3 groups：control，chitosan and chitosan-silk fibroin-TCP groups. Each group consisted of two dogs. The dog PDLCs cultured in vitro were collected. Then we took the 4th generation cells into the chitosan-silk fibroin-TCP. Then dogs were sacrificed after 8 weeks. The morphological changes were observed by HE staining. Cell proliferation was assayed using MTT method. Results    The result of HE staining shows that the periodontal space reduced. Growth and proliferation of dog PDLCs of chitosan-silk fibroin-TCP group was not significantly different from that of chitosan group. Conclusion    Chitosan-silk fibroin-TCP showed excellent adhesion and good biocompatibility with the dog PDLCs in vitro compared with the chitosan group. It is feasible.

Key words: periodontal ligament cells；chitosan；silk fibroin；tricalcium phosphate；tissue engineered