Expression and significance of FK506 binding protein 52 in chorionic villi of patients with recurrent spontaneous abortion.

doi:10.7504/fk2015060114

Abstract

Abstract:

Abstract： Objective To investigate the mRNA and expression of FK506 binding protein 52（FKBP52） in the chorionic villi of patients with recurrent spontaneous abortion（RSA） and normal women during early pregnancy.

Key words: recurrent spontaneous abortion, FKBP52, chorionic villi, RT-PCR, immunohistochemistry

摘要：

目的探讨复发性流产（RSA）病人及正常早孕妇女绒毛组织中FK506结合蛋白52（FKBP52）基因和蛋白的表达。方法　选取2012年2月至2013年6月广西医科大学第一附属医院妇产科门诊就诊的30例RSA妊娠妇女（复发性流产组）及同期正常早孕妇女30例（对照组），收集其新鲜绒毛组织，分别应用实时定量聚合酶链反应（RT-PCR）及免疫组织化学方法检测两组绒毛组织中FKBP52 基因和蛋白的表达。结果　 FKBP52在两组妇女的绒毛组织中均有表达，但与对照组相比，复发性流产组凝胶成像系统半定量分析FKBP52 mRNA（1.484 ± 0.198对1.109 ± 0.292，P<0.05）及蛋白表达（91.8 ± 5.3对68.2 ± 8.4，P<0.01）均明显降低。结论　 FKBP52的表达可能在胚胎着床和早期妊娠的维持中起重要作用，其表达降低可能与RSA的发病密切相关。

关键词: 复发性流产, FK506结合蛋白52, 绒毛组织, 实时定量聚合酶链反应, 免疫组织化学

CLC Number:

R714.21

CHEN Hong-yan*，LI Ou-yang，PANG Li-hong*，FAN Xiao-jing*，LIANG Hai-feng*，CHEN Xian-feng*.. Expression and significance of FK506 binding protein 52 in chorionic villi of patients with recurrent spontaneous abortion.[J]. Acta Metallurgica Sinica, DOI: 10.7504/fk2015060114.

陈红燕1，2，黎欧阳2，庞丽红1，范晓婧1，2，梁海峰1，2，陈先凤1，2. 复发性流产病人绒毛组织中FK506结合蛋白52的表达及意义[J]. 中国实用妇科与产科杂志, DOI: 10.7504/fk2015060114.

References

［1］   Practice Committee of the American Society for Reproductive Medicine.Definitions of infertility and recurrent pregnancy loss：a committee opinion［J］. Fertil Steril，2013，99（1）：63.

［2］   肖世金，赵爱民. 复发性流产病因学研究进展［J］. 中国实用妇科与产科杂志，2014，30（1）：41-45.

［3］   Alijotas-Reig J，Garrido-Gimenez C. Current concepts and new trends in the diagnosis and management of recurrent miscarriage［J］. Obstet Gynecol Surv，2013，68（6）：445-466.

［4］   Tranguch S，Smith DF，Dey SK. Progesterone receptor requires a co-chaperone for signalling in uterine biology and implantation［J］. Reprod Biomed Online，2006，13（5）：651-660.

［5］   Yang Z，Wolf IM，Chen H，et al. FK506-binding protein 52 is essential to uterine reproductive physiology controlled by the progesterone receptor a isoform［J］. Mol Endocrinol，2006，20（11）：2682-2694.

［6］   Smith ML，Schust DJ. Endocrinology and recurrent early pregnancy loss［J］. Semin Reprod Med，2011，29（6）：482-490.

［7］   Arredondo F，Noble LS. Endocrinology of recurrent pregnancy loss［J］. Semin Reprod Med，2006，24（1）：33-39.

［8］   Hinds TD，Stechschulte LA，Elkhairi F，et al. Analysis of FK506，timcodar （VX-853） and FKBP51 and FKBP52 chaperones in control of glucocorticoid receptor activity and phosphorylation［J］. Pharmacol Res Perspect，2014，2（6）：e00076.

［9］   Yong W，Yang Z，Periyasamy S，et al. Essential role for Co-chaperone Fkbp52 but not Fkbp51 in androgen receptor-mediated signaling and physiology［J］. J Biol Chem，2007，282（7）：5026-5036.

［10］   Tranguch S，Cheung-Flynn J，Daikoku T，et al. Cochaperone immunophilin FKBP52 is critical to uterine receptivity for embryo implantation［J］. Proc Natl Acad Sci USA，2005，102（40）：14326-14331.

［11］   Tranguch S，Wang H，Daikoku T，et al. FKBP52 deficiency-conferred uterine progesterone resistance is genetic background and pregnancy stage specific［J］. J Clin Invest，2007，117（7）：1824-1834.

［12］   Tranguch S，Smith DF，Dey SK. Progesterone receptor requires a co-chaperone for signalling in uterine biology and implantation［J］. Reprod Biomed Online，2007，14（1）：39-48.

［13］   Riggs DL，Roberts PJ，Chirillo SC，et al. The Hsp90-binding peptidylprolyl isomerase FKBP52 potentiates glucocorticoid signaling in vivo［J］. EMBO，2003，22（5）：1158-1167.

［14］   Haverinen M，Passinen S，Syvala H，et al. Heat shock protein 90 and the nuclear transport of progesterone receptor［J］. Cell Stress Chaperones，2001，6（3）：256-262.

（2015-02-24收稿    2015-04-22修回）

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