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06 May 2006, Volume 21 Issue 05 Previous Issue    Next Issue
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Rotavirus molecular epidemic features in infants with enteritis in Kunming from 2002 to 2004. Zhen Shuangping,Huang Yongkun,Hou Zongliu 2006, 21(05): 353-356 .  Abstract ( )   ObjectiveTo investigate molecular epidemic features of rotavirus in infants with enteritis in Kunming,China,2002~2004. MethodsStool samples were collected from 210 children who were admitted to the Department of Pediatric, the First Affiliated Hospital of Kunming Medical College from September to December from 2002 to 2004.Polyacrylamide gel electrophoresis(PAGE) was used to detect rotavirus genemic RNA and identify electropherotypes of group A rotavirus.Reverse transcription polymerase chain reaction(PTPCR) was performed to identify rotavirus G serotypes. ResultsOut of 210 samples investigated,143(68%) were group A rotavirus gene positive by PAGE, and these showed long electropherotypes;while none showed short electropherotypes or mixed electropherotypes.RTPCR were performed for 143 fecal specimens and VP7 gene products were obtained from 134(937%) samples.NestedPCR showed serotype G3 accouted for 545%(73)of these samples;serotype G1 accouted for 284%(38);coinfected by 2or 3 rotavirus with different G type accouted for 6%(8);only four G4 was detected and 11 were nonotypable;none was G2 or G9NSP4 gene products were obtained from 134 samples which gained VP7 gene positive. Out of 134 samples,30 gains NSP4 CDNA 500bp by nestedPCR the test of which showed variance. ConclusionGroup A rotavirus is the major pathogen for enteritis in infants in Kunming during the period of September to December from 2002 to 2004.Rotavirus with long electropherotype was dominant during these years and G3 was the most common serotype followed by G1,whereas serotype G4 was seldom found.No serotype G2 or G9 was found.Clinical manifestations is not related to the variation of NSP4.

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A study on the association between fetal growth retardation and the change of insulin sensitivity in the neonates. Liu Zhanli，Liang Kun，He Xiangying 2006, 21(05): 357-359 .  Abstract ( )   AbstractObjectiveTo explore the relationship between fetal growth retardation(FGR)and the change of insulin sensitivity in neonates. MethodsFrom April 2004 to December 2004,72 small for gestational age infants(SGA)and 48 appropriateforgestationalage infants(AGA)were selected in the present study in the First Affiliated Hospital of Kunming Medical College.Fasting plasma glucose(FPG),fasting insulin(FINS),high density lipoproteincholesterol(HDLC),low density lipoproteincholesterol(LDLC),triglyceride(TG) and nonesterified fatty acids(NEFA) were assayed;glucose/insulin ratio (G/I)、insulin sensitivity index(ISI)、insulin resistance index(HOMAIR)，the function of βcell of pancreatic islands(HCBI)，etc.were calculated.Explore the relationship between FGR and the change of insulin sensitivity in neonates. ResultsThe level of FPG and HDLC in SGA group was lower than that in AGA group，but the level of FINS、LDLC、TG and NEFA was higher(P<001).G/I ratio、ISI in SGA group were lower than that in AGA group.HOMAIR was higher(P<001).However，there was no significant difference in HBCI between two groups. ConclusionIn early postnatal life FGR neonates display decreased insulin sensitivity and insulin resistance (IR)to some extent.However，the function of βcell of pancreatic islands has no remarkabe change.

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The study of correlations between the contents of leptin in cord blood and body immune function. Jiang Lian,Li Huanan,Rong Xiaoping 2006, 21(05): 360-362 .  Abstract ( )   AbstractObjectiveThe main purpose of the study was to research into the correlation of cord blood leptin levels and neonates immunologic function as well as the possible immunomodulate mechanism. MethodsThirty preterm infants and 60 term infants of our hospital were selected from March to October in 2003,respectively.They were checked up the contents of leptin,GMCSF,IgG,IgA,IgM,C3 and C4 in cord blood serum,and the expression of IL4,IFNγ,bcl2 protein,T lymphocyte subpopulation and lymphocyte apoptosis percentage in cord blood by radioimmunoassay(RIA)、immunotransmission turbidity method and flowcytometry. ResultsThe umbilical vein blood leptin contents were positively correlated with the IgG (P<001)、C3 (P<001)、C4 (P<005) levels、the CD4+T lymphocyte percentage (P<005)、CD8+T lymphocyte percentage (P<001)、IFNγ levels (P<005)、GMCSF levels and the expression of bcl2 protein;there had negative correlations between the cord blood leptin levels and IL4 levels、lymphocyte apoptosis percentage;there had no significant correlation between leptin levels and IgA、IgM levels (P>005). ConclusionThe cord blood leptin levels are strongly correlated with neonates’ immune functions.Leptin could enhance the cellular immune function.But leptin has minor influence on humoral immunity.Leptin could raise the expression of bcl2 albumin and the contents of GMCSF,which is believed to be the possible important mechanisms of the leptin's immunomodulation function.

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Correlation between brain natriuretic peptide in blood plasma and hyponatremia in the newborn with hypoxia ischemic encephalopathy. Zhang Wei,Yu Xia,Liao Tingyan 2006, 21(05): 363-365 .  Abstract ( )   AbstractObjectiveTo studying the correlation between brain natriuretic peptide(BNP) in blood plasma and hyponatremia in hypoxia ischemic encephalopathy(HIE) of the newborn and the physiological and pathological mechanism of BNP and hyponatremia in the pathological course of HIE. MethodsObserve BNP in blood plasma and natrium level dynamically in 22 infants with HIE,including the age of 3 days,7days and 2 weeks,and analyse the correlation.In the mean time make a comparison between 52 infants with HIE with normal blood natrium and infants with nonHIE with normal blood natrium. ResultsBNP of infants with HIE aged 3 days was remarkably higher than the control group(P<001);in the 7 days aged,there was no obvious difference between the infants with HIE with normal blood natrium and the control group,while the hyponatremia group was still distinctly higher than the normal blood natrium group (P<001).The difference among BNP in the hyponatremia group was distinct in the three periods(P<001).There was a decrease of the blood natrium density in the hyponatremia group at every period.The ordinal comparison difference was distinct.The neonatal behavioral neurological assessment (NBNA) comparison difference in the relative period was remarkable (P<001) at the hyponatremia group and normal blood natrium group.NBNA comparison difference was remarkable (P<001) at the three periods for the infants with HIE in hyponatremia group.BNP level and blood natrium ion in the hyponatremia group had a negative relationship in each period. ConclusionBNP might cause and participate in hyponatremia in infants with HIE,and may play an important role in the pathogenesis of HIE directly or by hyponatremia intraduction.

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The reduced plasma glutamine concentration in infants with necrotizing enterocolitis. Chen Zhijuan，Huang Miaohui，Chen Hanqiang 2006, 21(05): 366-368 .  Abstract ( )   AbstractObjectiveTo determine the relationship between the plasma levels of glutamine(Gln) and NEC. MethodsWe chose sixteen infants who developed NEC from October 2002 to October 2003. We also chose sixteen nonNEC infants as the controls,who were of the same sex,age,correspond gestational age and birth weight.We measured the plasma levels of Gln of these infants and control ones by RPHPLC,and observed the plasma levels of Gln.Then we determined the relationship between the plasma levels of Gln and NEC after controlling the possible confounding bias by multivariate analysis. ResultsThe mean plasma level of Gln in NEC was 02108±00813mmol/L,which was significantly lower than controls (03490±01388 mmol/L)(P<005).Adjusted mean plasma levels and 95% confidence interval of Gln in NEC and control group were 0216 mmol/L(0150~0282mmol/L) and 0344 mmol/L(0278~0410mmol/L),respectively.There was significant difference between two groups(P=0032).The univariate χ2 analysis showed that variables:plasma level of Gln,hypoxia and infection,were significantly different between two groups;and the variables:plasma level of Gln and hypoxia,were the risk factors for NEC in conditional multiple regression. ConclusionInfants who developed NEC have reduced level of Gln,and the reduced level of Gln may predispose to the illness.

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The relationship between dopamine transporter allele 440bp and childhood autism. He Wen,Sun Xiaomian,Li Yamei. 2006, 21(05): 369-371 .  Abstract ( )   AbstractObjectiveTo investigate the relationship of dopamine transporter allele 440bp and childhood autism. MethodsAnalyzing the correlation of childhood autism among Chinese Han nationality and the gene polymorphism of dopamine transporter (DAT1) by PCR technique from March to August of 2004. Results(1)Five alleles (320bp，360bp，440bp，480bp，520bp )and 6 gene type(480/480，480/320，520/480，480/360，480/440,440/440)of DAT1 gene polymorphism were found.(2)The allele and gene type of CA and DAT were evaluated by relative hazard.The results showed that gene type 480/440 and allele 440 correlated significantly with CA,and their relative hazard were 265 and 23.They also showed that gene type 480/480 and allele 480 correlated negatively,but was statistical unsignificant,and their relative hazard was 064 and 077. ConclusionThere is no genetic correlation between DAT1440bp and childhood autism among Chinese Han nationality.The allele 440bp and gene type 480bp/440bp may be two risk factors,but it needs to be further investigated.

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Tissue inhibitors of metalloproteinase1 and progression muscular dystrophy Sun Guilian,Zhao Shuang，Jiang Hongkun 2006, 21(05): 372-375 .  Abstract ( )   AbstractObjectiveOur aim is to study the role of tissue inhibitor of metalloproteinase1 (TIMP1) in progressive muscular dystrophy (PMD). MethodsFrom Apr.2002 to Mar.2003,we examined the expression and cellular localization of TIMP1 protein using biopsied frozen muscle from patients with Duchenne muscular dystrophy(DMD)，Becker muscular dystrophy(BMD)，congenital muscular dystrophy (CMD) by immunohistochemistry，double immunofluorescence and Western blot analysis. ResultsThe results of immunohistochemistry and double immunofluorescence showed that TIMP1 was positive in vascular endothelial cells of normal muscles.Immunohistochemistry and Western blot analysis showed that the staining intensity was distinctly increased in dystrophic muscles of PMD for TIMP1.Double immunofluorescence revealed that TIMP1 strongly expressed in the regenerating muscle fibers，macrophages and macrophage infiltrating necrotic fibers.Some activated fibroblasts in endomysium and perimysium of DMD and CMD muscles were also positive for TIMP1. ConclusionThe elevated local production of TIMP1 in diseased muscles of PMD and their distinct distribution pattern provide evidence that TIMP1 may participate in the pathogenesis of PMD.

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Severe liver disease combined with spur cell anemia: a review of 7 cases. Zhu Chunjiang,Li Xiong，Zhang Ming 2006, 21(05): 376-379 .  Abstract ( )   AbstractObjectiveTo explore the clinical characteristics of severe liver disease combined with spur cell anemia in 7 children. MethodsThe clinical data of 7 patients with such disease admitted to the pediatric ward in our hospital between January 2001 to March 2005 were analyzed.The data included symptoms、 signs、 laboratory examinations ( blood smear、 liver function test and serum cholesterol ) and the literatures,etc. ResultsSeven patients consisted of 4 males and 3 females and the age ranged from 5 months to 9 years.The children manifested as jaundice(6 cases)、 hemorrhage (3 cases)、anemia(7 cases)、large liver(6 cases) or cirrhosis(1 case) and megalospleen(3 cases).Blood smear showed that spur cell was 85%~9438% of the whole erythrocytes in peripheral blood.Liver functions were destroyed badly.Serum cholesterols were abnormal. ConclusionThe clinical characteristics of the children are summed up as:although the original disease might be different,the children are all characterized as long course、moderate to mild anemia、spur cell in peripheral blood、destroyed liver function、abnormal plasma cholesterol and bad prognosis.