Abstract:

To study the levels of serum IL-6，IL-8，IL-1β，TNF-α，GMP-140and the number of platelet in different treatment for patients with Kawasaki disease， and to investigate the mechanism of TanshinoneIIA in Kawasaki disease. Methods　Sixty-one patients with

Kawasaki disease and nineteen healthy children as controls were recruited into the study. The patients with Kawasaki disease were randomly divided into two groups：TanIIA treatment group and contrast treatment group.The serum IL-6，IL-8，IL-1β，TNF-α and GMP-140 levels were detected by ELISE and peripheral blood platelet number was measured using automatic hematocyte analyzer. Results　The levels of serum IL-6， IL-8， IL-1β， TNF-α and GMP-140 were increased before treatment，and decreased after treatment of five to seven days.The difference was statistically significant.The number of platelet was slightly increased before treatment，and significantly increased after treatment of five to seven days. After treatment of five to seven days，the levels of serum IL-6， IL-1βand GMP-140 in the group of TanIIA treatment decreased significantly compared with those in the group of contrast treatment，and the difference was statistically significant. Concluson　TanIIA might diminish the damage of immune vasculitis and platelet activation by decreasing the levels of serum IL-6， IL-1βand GMP-140.

Key words:  , Kawasaki disease；inflammatory cytokines；GMP-140； platelet；TanshinoneIIA

摘要：

观察不同的治疗方案下川崎病（KD）患儿治疗前后血清白介素-6（IL-6）、白介素-8（IL-8）、白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、P-选择素（GMP-140）和血小板数量的变化，分析丹参酮IIA（TanIIA）对各指标的影响，探讨其作用机制。方法　2009年10月至2010年9月成都市儿童医院住院治疗的KD患儿61例。将住院KD患儿随机分为TanIIA加常规治疗组和常规治疗组，并与健康体检儿童作对照。采用双抗体酶联免疫吸附法（ELISA）检测治疗前后患儿血清IL-1β、IL-6、IL-8、TNF-α和GMP-140，用全自动血细胞分析仪检测外周血血小板计数。结果　KD患儿治疗前血清IL-6、IL-8、TNF-α、GMP-140和血小板数量均较正常对照组显著升高，治疗后5～7 d血小板数量明显升高，血清IL-6、IL-8、IL-1β、TNF-α、GMP-140显著降低，其中TanIIA加常规治疗组较常规治疗组血清GMP-140、IL-6、IL-1β下降更明显，差异有统计学意义（P < 0.05）。结论　TanIIA可以一定程度地降低KD患者血清IL-6、IL-1β、GMP-140，从而减轻血管炎性损伤和血小板的活化。

关键词: 川崎病, 炎性细胞因子, GMP-140, 血小板, 丹参酮IIA