中国实用儿科杂志

中国实用儿科杂志 ›› 2025, Vol. 40 ›› Issue (5): 413-417.DOI: 10.19538/j.ek2025050613

• 论著 • 上一篇    下一篇

呼吸道合胞病毒感染p38MAPK水平与Th1和Th2细胞因子相关性研究

  

  1. 1.湖南省人民医院 湖南师范大学附属第一医院儿童医学中心，湖南  长沙  410005；2.湖南省疾病预防控制中心，湖南  长沙  410005
  • 出版日期:2025-05-06 发布日期:2025-08-28
  • 通讯作者: 钟礼立，电子信箱：zhonglili1@hunnu.edu.cn
  • 基金资助:
    湖南省卫生健康委国家临床重点专科重大科研专项项目（Z2023054）；湖南省人民医院仁术基金项目（RS2022B03）

Correlation between p38MAPK level and Th1 and Th2 cytokines in RSV infection

  1. *Children's Medical Center，Hunan Provincial People's Hospital and First Affiliated Hospital of Hunan Normal University，Changsha  410005，China
  • Online:2025-05-06 Published:2025-08-28

摘要： 目的    描述呼吸道合胞病毒（RSV）中住院治疗患儿临床特征及初步探讨p38丝裂素活化蛋白激酶（p38MAPK）表达水平与Th1和Th2细胞因子的相关性。方法    选取2023年1月1日至2023年6月30日在湖南省人民医院住院进行鼻咽拭子检查的154例RSV感染患儿为RSV组，并进一步根据是否有湿疹、荨麻疹、过敏性鼻炎及相关过敏性疾病家族史等将154例RSV感染患儿分为特应征组（74例）和非特应征组（80例）。提取整理入组患儿的基本情况、实验室检查报告等，细胞实验分为对照组、RSV组及RSV+SB202190（p38MAPK抑制剂）组，以实时荧光定量PCR法检测p38MAPK mRNA水平及ELISA法检测部分细胞因子表达。并通过ELISA法测定p38MAPK蛋白水平及采用流式细胞因子微球检测法测定Th1和Th2等细胞因子水平。结果    相对于对照组，RSV组P38MAPK mRNA水平及白细胞介素（IL）-4、IL-5、干扰素（IFN）-γ的分泌明显增加（P<0.05）。而相对于RSV组，RSV+SB202190组P38MAPK mRNA的表达及IL-4、IL-5、IFN-γ的分泌量显著减少（P<0.05）。与非特应征组比较，特应征组嗜酸性粒细胞（EOS）计数、免疫球蛋白A（IgA）、白细胞介素（IL）-4、、IL-4/IFN-γ、p38MAPK水平及IgE升高比例明显增多（P<0.05）。而特应征组较非特应征组IFN-γ水平明显降低（P<0.05）。进一步相关分析提示p38MAPK与IL-4、IL-4/IFN-γ呈正相关（rs分别为0.58、0.68，P<0.05）。结论    p38MAPK可能通过调控Th1、Th2细胞因子水平，参与RSV呼吸道感染的发生发展。

关键词: 呼吸道合胞病毒, p38MAPK, 细胞因子

Abstract: Objective    To investigate the clinical features of hospitalized children with respiratory syncytial virus（RSV）and the correlation between the expression of p38MAPK and type I helper T cell（Th1）and type Ⅱ helper T cell（Th2）cytokines. Methods    A total of 154 children with RSV infection who were hospitalized in Hunan Provincial People's Hospital from January1，2023 to June 30，2023 and underwent nasopharyngeal swab examination were selected as RSV group. According to the family history of eczema，urticaria，allergic rhinitis and related allergic diseases，the 154 children with RSV infection were further divided into atopic group（74 cases）and non-atopic group（80 cases）.Extract and organize the general data and the laboratory examination reports of the children included. In the cell experiment there were divided into control group，RSV group，and RSV+SB202190（p38MAPK inhibitor）group. Real time fluorescence quantitative PCR was used to detect p38MAPK mRNA levels，and ELISA was used to detect partial cytokine expression. Results    Compared to the control group，the RSV group showed a significant increase in P38MAPK mRNA levels and secretion of IL-4，IL-5，and IFN-γ（P<0.05）. Compared with the RSV group，the expression of P38MAPK mRNA and the secretion of IL-4，IL-5，and IFN-γ were significantly reduced in the RSV+SB202190 group（P<0.05）. Compared with the non-atopic group，the atopic group had significant increases in eosinophil（EOS）count，immunoglobulin A（IgA），interleukin-4（IL-4），IL-4/IFN-γ，p38MAPK and IgE（P<0.05）. The atopic group had a significantly lower level of IFN-γ than the non-atopic group（P<0.05）. Further correlation analysis showed that p38MAPK was positively correlated with IL-4 and IL-4/IFN-γ（rs=0.58，0.68，P<0.05）. Conclusion    The p38MAPK may be involved in the development of RSV respiratory tract infection by regulating the levels of Th1 and Th2 cytokines.

Key words: respiratory syncytial virus, p38MAPK, cytokines