Abstract:

Abstract：Objective To investigate the role and relationship between BMP-1 and Noggin in bone formation. Methods Establish two types of orthodontic tooth movement models using incisors and implants as the anchorage part respectively. Measure the distance of tooth movement. The tissue was stained with HE to observe the histological changes of periodontal tissues. Immunohistochemical assay was used to determine BMP-1 and Noggin levels. And the result was analyzed by statistic test. Results The distance of tooth movement between two models was different. BMP-1 and Noggin had the same temporal and spatial expression profiles during the orthodontic tooth movement. The expression of them were clearly upregulated in the early phase and then decreased. The expressions of them at the tension side were much more obvious than the pressure side. They reached the peak on day 7，then decreased gradually，and the expression was almost equal to the control group on day 28. Conclusion The tooth movement model using implant as the anchorage is better than the traditional one. BMP-1 and Noggin are both involved in the rat bone remodeling，and play a very important role.

Key words: tooth movement, bone remodeling, implant anchorage, BMP-1, Noggin

摘要：

目的 探讨牙齿移动过程中骨形态发生蛋白（BMP）-1与Noggin在牙周组织中的表达及作用。方法 2010年7月至2011年3月在大连医科大学附属第二医院分别建立以大鼠切牙及种植体为支抗的正畸牙齿移动模型，测量磨牙移动距离，用苏木精—伊红（HE）染色观察被移动磨牙牙周组织形态学变化，应用免疫组化方法检测BMP-1与Noggin在牙周组织改建过程中的表达情况。结果 以种植体为支抗的正畸牙齿移动模型，其磨牙的移动距离和速度优于以切牙为支抗组。BMP-1与Noggin在牙周组织的分布及表达在空间和时间上具有明显的一致性：均表现为实验组表达明显强于对照组，张力侧表达强于压力侧；二者在牙周组织中的表达水平均在第7天组最强，到第28天组便基本接近对照组的水平。结论 以种植体为支抗的正畸牙齿移动模型优于传统方法。BMP-1与Noggin均参与了牙周组织的改建过程，且可能在该过程中发挥重要的调节作用。

关键词: 牙齿移动, 骨改建, 种植体支抗, BMP-1, Noggin