Abstract:

Objective    To investigate the influence of Bushen Zhuanggu Decoction on the bone healing after teeth extraction in osteoporotic dogs. Methods    Totally 9 female adult dogs whose bilateral ovaries were surgically removed to establish osteoporosis models were randomly divided into 3 groups （control group，Western medicine group and traditional Chinese medicine group）. Bilateral mandibular premolars were extracted 3 months after castration. Osteoporosis treatment was started the third day after tooth extraction. The dogs in the traditional Chinese medicine group，the Western medicine group and the control group were respectively fed with Bushen Zhuanggu Decoction，Estradiol Valerate Tablets and nothing. The bone mineral density（BMD） value of dog′s lumbar spine and mandible of each group were detected before and after 3 months of castration and 3 months after teeth extraction. The tissue morphology of the mandible was observed at 1，2 and 3 months after teeth extraction. Results    Three months after teeth extraction，the BMD values of the lumbar spine and mandible in the traditional Chinese medicine group and the Western medicine group were significantly increased compared with those before the osteoporosis treatment，and there was no significant difference compared with the preoperative ones. One month after teeth extraction， the new bone and the trabecular bone were observed in irregular patterns in the control group and the Westen medicine group，and those were arranged in a mesh pattern in the Chinese medicine group. Two months after teeh extraction，the trabecular bone structure was sparse in the control group；the Western medicine group was more than that of the new bone，and the number of trabecular bone in the Chinese medicine group was more robust. Three months after teeth extraction，the bone trabecular structure was relatively compact in the control group and the gap is small；the new bone tissue and the original bone tissue roughly fused together in the Western medicine group；the new bone tissue of the traditional Chinese medicine group was more similar to the structure of the original bone. Conclusion      Bushen Zhuanggu Decoction can make cancellous bone around the teeth extraction of the osteoporosis canine and significantly increase the amount of bone tissue，so as to promote bone reconstruction and bone healing after teeth extraction.

Key words: Bushen Zhuanggu Decoction, osteoporosis, bone healing, bone mineral density

摘要：

目的    探讨补肾壮骨合剂对骨质疏松犬拔牙后骨愈合的影响。方法    选择3岁龄杂种雌性成年犬9只，随机均分为对照组、西药组和中药组。手术切除9只犬的双侧卵巢（去势术），检测腰椎骨密度（BMD）以确定建立骨质疏松模型。各组犬在去势术后3个月拔除两侧下颌前磨牙，拔牙术后第3天开始骨质疏松治疗，中药组灌服补肾壮骨合剂溶液，西药组服用戊酸雌二醇片（补佳乐），对照组不进行任何治疗。在去势术前、去势术后3个月以及拔牙后3个月分别检测各组犬的腰椎BMD和下颌骨BMD，并于拔牙术后1、2、3个月切取下颌骨进行组织形态学观察。结果    拔牙后3个月，中药组、西药组的腰椎和下颌骨BMD较骨质疏松治疗前显著提高，与去势术前比较差异均无统计学意义（均P > 0.05）；而对照组拔牙后3个月的腰椎和下颌骨 BMD未见明显改善，仍明显低于去势术前（P < 0.05）。下颌骨组织形态学观察显示，拔牙后1个月，对照组、西药组拔牙创愈合的新骨小梁走行不规则；中药组新生骨小梁排列成网状。拔牙后2个月，对照组局部骨小梁结构较稀疏；西药组新生骨小梁粗壮，排列较整齐；中药组新生骨骨小梁呈网状结构，骨小梁数量多、粗壮。拔牙后3个月，对照组拔牙创骨小梁结构比较致密，间隙较小；西药组新生骨组织与原骨组织大致融合一体；中药组新骨组织与原骨组织结构基本一致。结论    补肾壮骨合剂治疗可使骨质疏松犬拔牙创周围松质骨区骨量明显增加，有助于骨改建，有效促进拔牙创骨愈合。

关键词: 补肾壮骨合剂, 骨质疏松, 骨愈合, 骨密度