中国实用口腔科杂志

中国实用口腔科杂志 ›› 2012, Vol. 5 ›› Issue (08): 473-475.

• 论著 • 上一篇    下一篇

大鼠髁状突颈部骨折对软骨细胞上游刺激因子1表达影响研究

  

  1. 1.云南陆良县人民医院口腔科，云南  陆良  655600；2.昆明医科大学口腔医学院，昆明  650031；3.云南省边防总队医院口腔科，昆明  650000
  • 收稿日期:2012-04-07 修回日期:2012-07-11 出版日期:2012-08-15 发布日期:2012-09-14

  • Received:2012-04-07 Revised:2012-07-11 Online:2012-08-15 Published:2012-09-14

摘要： 目的    研究发育期SD大鼠髁状突颈部骨折对下颌髁状突软骨细胞中上游刺激因子1（USF1）表达的影响。方法    本研究于2011年7—9月在昆明医科大学口腔医学院研究所完成。选择4周龄雄性SD 大鼠12只，其中6只大鼠用于制作下颌单侧髁状突颈部骨折动物模型，6只大鼠为对照。分别在术后1、3、5周时处死大鼠，取出骨折侧、骨折对侧以及空白对照的髁状突软骨。采用免疫组织化学方法检测髁状突软骨细胞中USF1表达情况。结果    术后1周，骨折对侧空白对照组与骨折侧的USF1表达有显著性差异（P＜0.01）；术后3周，空白对照组与骨折侧USF1表达有显著性差异（P＜0.01）。骨折侧术后3周、5周与术后1周USF1表达有显著性差异（P＜0.01）。结论    单侧髁状突颈部骨折引起应力变化，使患侧髁状突软骨细胞中USF1表达异常，进而影响髁状突的生长发育。

关键词: 骨折, 髁状突软骨, 上游刺激因子1, 大鼠, 软骨细胞

Abstract: Objective    To investigate the effect of unilateral condylar neck fracture on USF1 expression of the chondrocyte in growing rats. Methods    Twelve 4-week-old rats were randomly divided into two parts：half were operated to cause condylar fracture，the other part as control. The rats were killed at 1，3 and 5 weeks after surgery，and the condylar cartilage was obtained from the operating side and non-operating side of experimental group and control group. Immunohistochemical staining was used to qualitively examine USF1 expression. Results    There was significant difference between non-operating side，control group and opetating side at 1 week after surgery. There was significant difference between operating side and control group at 3 weeks after surgery. There was significant difference between 3 and 5 weeks and 1 week after surgery on operating side. Conclusion    The unbalanced stress caused by unilateral condylar neck fracture change USF1 expression affect the development and growth of condyle.

Key words: fracture, condylar cartilage, USF1, rat, chondrocyte