中国实用口腔科杂志

中国实用口腔科杂志 ›› 2012, Vol. 5 ›› Issue (04): 210-213.

• 论著 • 上一篇    下一篇

牙龈卟啉单胞菌对人牙周膜成纤维细胞MMP-1和TIMP-1表达影响的研究

  

  1. 中国医科大学口腔医学院 a牙周科，b中心实验室，沈阳  110002
  • 收稿日期:2012-01-21 修回日期:2012-03-22 出版日期:2012-04-15 发布日期:2012-05-16

  • Received:2012-01-21 Revised:2012-03-22 Online:2012-04-15 Published:2012-05-16

摘要：

目的    探讨牙龈卟啉单胞菌（Porphyromonas gingivalis，P. gingivalis）W83、ATCC33277刺激人牙周膜成纤维细胞（HPDLFs）分泌基质金属蛋白酶-1（MMP-1）、金属蛋白酶组织抑制剂-1（TIMP-1）的变化。方法    本研究于2010年9月至2011年6月在中国医科大学口腔医学院中心实验室进行。将P. gingivalis W83、ATCC33277作用于HPDLFs 0、6、12、24、48 h后，运用酶联免疫吸附法（ELISA）检测细胞上清液中MMP-1、TIMP-1质量浓度变化，并计算MMP-1/TIMP-1值。结果    P. gingivalis感染HPDLFs的MMP-1和TIMP-1表达均增强，并呈时间依赖性；且MMP-1/TIMP-1值明显高于未感染的对照组（P < 0.05）。P. gingivalis W83感染后MMP-1/TIMP-1值明显高于P. gingivalis ATCC33277（P < 0.05）。结论    P. gingivalis具有促进HPDLFs分泌MMP-1、TIMP-1的作用，且可造成牙周组织破坏；P. gingivalis W83降解细胞外基质的能力高于P. gingivalis ATCC33277。

关键词: 牙周炎, 牙龈卟啉单胞菌, 基质金属蛋白酶-1, 金属蛋白酶组织抑制剂-1

Abstract:

Objective    To investigate the effects of Porphyromonas gingivalis on the expression of MMP-1 and TIMP-1 in human periodontal ligament fibroblasts through using an in vitro model. Methods    MMP-1 and TIMP-1 protein levels in culture supernatant were determined by ELISA at the different time intervals （6 h，12 h，24 h，48 h） following continuous co-culture of P. gingivalis W83 and ATCC33277 with HPDLFs. The ratio of MMP-1 / TIMP-1 was calculated. Results      The expression of MMP-1 and TIMP-1 was enhanced in HPDLFs infected with P. gingivalis；the ratio of MMP-1/TIMP-1 in infected HPDLFs was significantly higher than that of control group （P < 0.05）. The ratio of MMP-1/TIMP-1 in HPDLFs infected with P. gingivalis W83 was significantly higher than that in HPDLFs infected with P. gingivalis  ATCC33277 （P < 0.05）. Conclusion    P. gingivalis can accelerate secretion of MMP-1 and TIMP-1 in HPDLFs， which causes periodontal tissue destruction. P. gingivalis W83 has stronger pathogenicity for periodontal disease by producing extracellular matrix in local.

Key words: periodontitis；Porphyromonas gingivalis；MMP-1；TIMP-1