中国实用口腔科杂志

中国实用口腔科杂志 ›› 2010, Vol. 3 ›› Issue (05): 292-294.

• 论著 • 上一篇    下一篇

RECK和MMP-2在口腔鳞癌中的表达及其与侵袭转移关系研究

  

  1. 1.中国医科大学生物化学与分子生物学教研室，沈阳  110001；2.中国医科大学英语教研室，沈阳  110001
  • 收稿日期:2010-03-10 修回日期:2010-04-20 出版日期:2010-05-15 发布日期:2010-06-01

  • Received:2010-03-10 Revised:2010-04-20 Online:2010-05-15 Published:2010-06-01

摘要：

目的    探讨半胱氨酸丰富蛋白Kazal基元（RECK）及基质金属蛋白酶-2（MMP-2）的表达与口腔鳞癌发生、发展及侵润、转移的关系。方法     选择中国医科大学附属口腔医院病理科存档中2008年9月至2009年10月石蜡包埋的口腔鳞癌术后标本68例为实验组，对应的远端正常口腔黏膜组织68例为对照组。应用免疫组化SP法检测RECK及MMP-2蛋白表达，并在显微镜下观察阳性细胞所占百分比及着色深浅进行结果判定。结果    癌组织中RECK蛋白的阳性表达率为40.4%，明显低于正常口腔黏膜组织83.8% （χ2 =11.284，P < 0.01）。RECK的表达与组织学分级、侵润深度、淋巴结转移相关（P均 < 0.05）。癌组织中MMP-2蛋白的阳性表达率为76.5%，明显高于正常口腔黏膜组织27.9%（P < 0.01）。MMP-2蛋白阳性表达随着组织学分级、侵润深度级别的增加逐渐升高（P均 < 0.05）；有淋巴结转移的MMP-2蛋白阳性表达率高于无淋巴结转移组（P < 0.05）。RECK蛋白表达与MMP-2蛋白表达呈负相关关系（r=-0.5426）。结论    RECK和MMP-2在口腔鳞癌的侵润、转移过程中具有重要作用，RECK及MMP-2的联合检测可望成为口腔鳞癌早期诊断和判断预后的分子指标。

关键词: RECK, 基质金属蛋白酶-2, 口腔鳞癌, 侵润, 淋巴结转移

Abstract:

Objective    To investigate the expression of RECK and MMP-2 protein and their correlations with the occurrence，development，invasion and metastasis of oral squamous cell carcinoma. Methods    SP immunohistochemistry method was used to detect the expression of RECK and MMP-2 protein in 68 cases of oral squamous cell carcinoma and 68 cases of oral mucosa. Statistical analysis was performed using chi-square test. Results    The protein expression of RECK and MMP-2 were closely correlated with the tumor grade， infiltration and lymph node metastasis in oral squamous cell carcinoma（all P < 0.05）. The expression rate of RECK protein was increased in tumor and the oral mucosa， which was 40.4% and 83.8%， respectively， and there was significant difference between every two groups（P < 0.01）；however， the expression rate of MMP-2 protein was decreased in tumor and the oral mucosa， which was 76.5%，27.9%， respectively， and there was significant difference between every two groups （P < 0.01）. Conclusion    RECK and MMP-2 may play important roles in the infiltration and metastasis of mucosal epithelium in oral squamous cell carcinoma. United detection of RECK and MMP-2 can be used in the early diagnosis and prognosis judgement of oral squamous cell carcinoma.

Key words: RECK；Matrix metalloproteinases-2；oral squamous cell carcinoma；invasion；lymph node metastasis