中国实用口腔科杂志

中国实用口腔科杂志

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光动力疗法治疗牙周病模型大鼠效果观察

栾秀玲1，马卫东1，秦艳利2，毕良佳3   

  1. 1. 大连市口腔医院，辽宁 大连 116021;2. 哈尔滨工业大学物理系，黑龙江 哈尔滨 150001;3. 哈尔滨医科大学附属第四医院口腔科，黑龙江 哈尔滨 150001

  • 收稿日期:2009-08-02 修回日期:2009-09-21 出版日期:2009-10-15 发布日期:2009-10-15

  • Received:2009-08-02 Revised:2009-09-21 Online:2009-10-15 Published:2009-10-15

摘要：

摘要：目的 探讨光动力疗法（PDT）对牙周病模型大鼠的治疗效果。方法 2006年5—8月在哈尔滨医科大学对16只大鼠上颌第一磨牙用细菌结扎线建立牙周病模型。6周后，将建模成功的16只大鼠随机分为对照组（8只）、实验组（8只），分别施以洁治刮治、光动力（1mg／mL甲苯胺蓝+12J／cm2二极管激光）治疗。每只鼠左侧进行治疗，右侧不进行治疗（阴性对照）。观察并比较两组治疗效果。结果 治疗后两组细菌均明显减少， 细菌菌落数从（1683±7.79）个、（1580 ±11.17）个分别减少为（67.32±6.06）个和（67.34±5.48）个（均P＜0.001）。两种方法治疗后细菌菌落数之间差异无统计学意义。光动力组和洁治组的对照组，其菌斑指数（PI）和牙龈出血指数（BI）分别为2.6±1.1和2.3±1.0;而对于光动力组和洁治组的治疗组PI和BI分别为1.9±0.8和1.8±0.7。病理切片显示两种治疗方法均取得相似的治疗效果。结论 光动力疗法能够有效治疗牙周病。

关键词: 牙周病, 光动力疗法, 洁治和刮治

Abstract:

Abstract：Objective To investigate the effect of photodynamic therapy in treating periodontitis in rats. Methods Periodontitis was developed by submerging ligatures at the subgingival region of maxillary molars in 16 rats. Six weeks later， the infection sites were treated either with 1 mg／mL of toluidine blue plus 12 J／cm2 red laser irradiation， or by routine scaling and root planing. The therapeutic efficacy was assessed by evaluating the reduction of total bacterial flora and histological changes of periodontal tissues. Results Significant reduction of total bacterial flora was achieved by both photodynamic therapy and conventional therapy. The signs of inflammation that accompanied periodontitis were greatly reduced without any obvious detectable injury to host tissues. Both photodynamic therapy and conventional therapy showed similar therapeutic results. Conclusion Toluidine blue-mediated photodynamic therapy could effectively treat periodontitis in vivo and has high potential in clinical application

Key words: periodontitis, photodynamic therapy, scaling and root planing