关键词: 成纤维细胞, 共培养, 口腔癌细胞

Abstract:

Objective To study the effect of fibroblasts on the growth of oral cancer cells and explore the roles of surviving in oral cancer angiogenesis. Methods We cultivated normal oral mucosa connective tissues （fibroblast） and epithelial cells （oral squamous carcinoma established cell line） together，using fibroblasts culture medium to cultivate tumor cells to observe the effect of fibroblast on the proliferation capacity of tumor cells. Results The result proved that under certain conditions （low blood serum），co-cultured fibroblast and tumor cells together could accelerate the growth of tumor cells. The fibroblast culture medium without blood serum had no obvious effect on the growth of tumor cells. Conclusion Under certain conditions （low blood serum），the cytokine excreted by the fibroblasts can promote the growth of the tumor cells. The fibroblasts culture medium has no influence on the growth of the tumor cells.

Key words: fibroblasts, co-culture, oral cancer cells