中国实用口腔科杂志

中国实用口腔科杂志

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高压氧暴露对富血小板纤维蛋白形态学作用研究

陈铁楼1a，张新海1a，梁曦嘉1b，方以群2，王世峰2，韩利华1c，徐东升3，唐大海1c，徐尔理1b，季颖颖1a   

  1. 1. 海军军医大学第一附属医院a全军口腔中心牙周科，b内分泌科, c检验科，上海  200081；2. 海军军医大学海军医学研究所高气压医学重点实验室，上海  200433；3. 上海宝山区中西医结合医院口腔科，上海  201999
  • 出版日期:2019-01-15 发布日期:2019-01-17
  • 基金资助:

    上海市卫计委面上项目（20134418）；中国人民解放军总后勤部面上项目（CHJ13J035）

  • Online:2019-01-15 Published:2019-01-17

摘要：

目的　探讨高压氧（hyperbaric oxygen，HBO）暴露对富血小板纤维蛋白（platelet-rich fibrin，PRF）的形态学影响。方法　选30例健康志愿者抽血制备PRF，均分为3组，试验1组PRF用0.25 MPa HBO暴露1 h，试验2组用0.25 MPa HBO暴露0.5 h，对照组不用HBO暴露，分别用光学显微镜、扫描电镜（SEM）和透射电镜（TEM）观察并比较3组形态学差异。结果　光学显微镜观察发现：试验1组胶原纤维染为红色，纤维排列致密，血小板多；试验2组纤维排列稍致密，血小板量较多；对照组纤维排列稀疏，血小板最少。SEM观察发现：试验1组纤维中多个血小板伸出突起，位于纤维表面或纤维之间；试验2组致密的胶原纤维交错排列，血小板伸出突起；对照组胶原纤维排列成网状，包裹多个静止的血小板。TEM观察发现：试验1组血小板内α-颗粒释放后成空泡状，在纤维蛋白或细胞之间散在颗粒样物质，白细胞中线粒体和粗面内质网增多；试验2组较多血小板α-颗粒释放，血小板内见空泡样结构；对照组血小板内α-颗粒处于激活或静止状态。结论　HBO对PRF中血小板激活有促进作用，使血小板α-颗粒释放生长因子于网状排列的胶原纤维中，发挥生物学作用。

关键词: 高压氧, 富血小板纤维蛋白, 形态学, 扫描电镜, SEM, 透射电镜, TEM

Abstract:

Objective　To investigate the effects of hyperbaric oxygen（HBO）exposure on the morphology of platelet-rich fibrin（PRF）. Methods　Thirty healthy volunteers were divided into 3 groups. The PRF of test group 1 was exposed to 0.25 MPa HBO for 1 hour. The test group 2 was exposed to 0.25 MPa HBO for 0.5 h，while control group was not exposed to HBO. The morphological differences were compared respectively among the 3 groups by light microscopy，scanning electron microscopy（SEM）and transmission electron microscopy （TEM）. Results　Light microscope showed that the collagen fiber was stained red，the fibers were compact，and the fibers contained more platelets in test group 1. In test group 2，the fiber arrangement was relatively compact and the amount of platelets was relatively more. In the control group，collagen fibers were arranged loosely and platelet count was the least. SEM observation found that many platelets protruded in the fibers in test group 1，which were located on the surface of the fibers or between the fibers. In test  group 2，dense collagen fibers were interlaced and platelets protruded. The collagen fibers in the control group were arranged in a reticular form and embedded several stationary platelets. TEM showed that the α-particles in platelets of group test 1 were released and became vacuoles，which dispersed between fibrin or cells，and the mitochondria and rough endoplasmic reticulum in white blood cells increased. In the test group 2，quite a few platelet α-particles were released and vacuolar structure was found in the platelets. The α-particles in platelet of the control group were in the active or stationary state. Conclusion　HBO can promote the activation of platelets in PRF and make α-particles of platelets release growth factor into the reticular collagen fibers to play its biological role.

Key words: hyperbaric oxygen；platelet-rich fibrin；morphology；scanning electron microscope, SEM；transmission electron microscope, TEM