中国实用口腔科杂志

中国实用口腔科杂志

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纳米珍珠粉对小鼠骨髓间充质干细胞活性影响研究

郑斌，徐普，李晓妮，陈灵，程亚楠，张文柏，王秋子   

  1. 中南大学湘雅医学院附属海口医院·海南省口腔医学中心口腔种植科，海南 海口570208（第一作者现工作于湖南省长沙市第一医院口腔科）
  • 出版日期:2019-01-15 发布日期:2019-01-17
  • 基金资助:

    海南省重点研发项目（ZDYF2016018）；海口市重点科技计划项目（2013-58）

  • Online:2019-01-15 Published:2019-01-17

摘要：

目的    探讨纳米珍珠粉（nano-pearl powder，NPP）对小鼠骨髓间充质干细胞活性的影响。方法    研究于2016年8月至2017年3月在中南大学湘雅医学院附属海口医院实验中心进行。 将NPP以100、300、500 μg/mL的质量浓度与小鼠骨髓间充质干细胞共培养24 h（NPP组），用CCK8法测定吸光度值，计算细胞相对增殖率，评定其细胞毒性级别；用Annexin-FITC/PI法检测细胞凋亡率。两者综合评定NPP对小鼠骨髓间充质干细胞活性的影响，并与相同质量浓度纳米羟基磷灰石（nano-hydroxyapatite，NHA）（NHA组）进行对比。结果    NPP与NHA细胞毒性检测均合格，NPP在100 μg/mL时细胞相对增殖率最高；NPP组细胞凋亡率均低于NHA组，在质量浓度为100和500 μg/mL时的差异有统计学意义（P < 0.01）。结论    NPP细胞毒性检测合格，其对小鼠骨髓间充质干细胞活性的影响优于NHA。

关键词: 纳米珍珠粉, 纳米羟基磷灰石, 骨髓间充质干细胞, 细胞活性

Abstract:

Objective    To investigate the effect of nano-pearl powder（NPP） on cell viability of mouse bone marrow mesenchymal stem cells. Methods    Mouse bone marrow mesenchymal stem cells（mBMMSCs）were co-cultured with NPP at concentration of 100，300，500 μg/mL for 24 h （NPP group）. CCK8 test was carried out to evaluate the relative growth rate and cytotoxicity. The apoptosis rate of cells was measured by Annexin-FITC/PI method. The effect of NPP on mBMMSCs activity was analyzed by the above two approaches，and the results were compared with nano-hydroxyapatite（NHA group）at the same concentration. Results    The cytotoxicity tests of NPP and NHA were qualified，and the relative proliferation rates of mBMMSCs in NPP groups were highest at 100 μg/mL. The apoptotic rates of mBMMSCs in NPP groups were all lower than those in NHA groups. The difference was statistically significant at 100 and 500 μg/mL（P < 0.01）. Conclusion    The cytotoxicity tests of NPP are qualified，and its effect on the cell viability of mBMMSCs is better than that of NHA.

Key words: nano-pearl powder, nano-hydroxyapatite, bone marrow mesenchymal stem cell, cell viability