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02 January 2015, Volume 31 Issue 1 Previous Issue    Next Issue

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Pay attention to the quality and safety of ovulation induction. QIAO Jie，ZHANG Yao-yao,LI Rong. 2015, 31(1): 13-14.  DOI: 10.7504/fk2014120102 Abstract ( )   PDF (933KB) ( )   Abstract: Ovulation induction is a key step to the successful pregnancy in assisted reproductive treatment.with a large number of application, drug selection, risks and complications get more attention. More large sample and multi-center clinical trials and animal experiments in this area were needed, so as to guide the rational clinical application of ovulation induction.

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The assessment of ovarian reserve and ovarian response. HU Lin-li, SUN Ying-pu. 2015, 31(1): 18-21.  DOI: 10.7504/fk2014120103 Abstract ( )   PDF (968KB) ( )   Abstract:The success rates of any artificial reproduction techniques depend on a correct protocol for ovarian stimulation. This can be decided only by proper assessment of ovarian reserve and ovarian response before commencing ovarian stimulation, including age, basal hormonal biomarkers, AFC, AMH,etc. But there is no specific marker which can independently assess ovarian reserve and ovarian response, and the exact assessment should be through combined application markers.

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The effect of controlled ovarian stimulation on endometrial receptiveity during the assisted reproductive process. JIN Li, PAN Jie-xue, DING Guo-lian, HUANG He-feng. 2015, 31(1): 22-25.  DOI: 10.7504/fk2014120107 Abstract ( )   PDF (739KB) ( )   Abstract: Despite many advances in assisted reproduction techniques, implantation rates are still low after in vitro fertilization (IVF) and controlled ovarian stimulation. Successful implantation and pregnancy require a vital embryo and an effective molecular dialogue with a ‘receptive’ endometrium. Endometrial receptivity is the rate-limiting step for the success of IVF. Ovarian stimulation is used in IVF programs to collect multiple oocytes and to produce multiple embryos per cycle of treatment. Although this procedure enables selection of high quality embryos for transfer, ovarian stimulation also results in supraphysiological levels of P and E2. These elevated sex steroid concentrations may impair endometrial receptivity. We try to evaluate the influence of COH on endometrial receptivity from ultrasonic imaging, morphocytology, histomorphology, molecular biology, and gene microarray analysis.

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Timing of HCG trigger and choice of triggering-drugs during ovulation induction. SHI Xiao, QUAN Song. 2015, 31(1): 24-28.  DOI: 10.7504/fk2014120105 Abstract ( )   PDF (996KB) ( )   Abstract:HCG trigger is important for inducing oocyte maturation and ovulation induction, which has also been thought one of the key points of ovulation induction and is closely related to the outcomes of ovulation induction.The timing of HCG trigger mainly depends on follicular diameter, serum estrogen and endometrial situation during ovulation induction. HCG and GnRH-a can be used for triggering during ovulation induction. The reasonable choice of triggering-drugs should be made according to different patients and ovulation induction protocols，which will be great helpful to improve the outcomes and decrease the complications.

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The effect of controlled ovarian stimulation on human oocytes. HAN Shu-biao, LIU Jun-xia, HUANG Guo-ning. 2015, 31(1): 29-31.  DOI: 10.7504/fk2014120106 Abstract ( )   PDF (954KB) ( )   Abstract: Controlled ovarian stimulation (COS) is one of the important ways in assisted reproductive technology，COS could get greater number of oocytes and enable multiple embryo transfer to increase clinical outcomes. But the oocytes are exposed to a totally different environment in COS，which may decrease the quality of oocyte. This paper reviews the effect of controlled ovarian stimulation on quality of oocyte, nuclear and cytoplasm maturity,metabolism of ATP generation, epigenetic modification and so on, in order to provide the reference for clinical controlled ovarian stimulation treatment.

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The effect of controlled ovarian stimulation on endometrial receptivity during the assisted reproductive process. JIN Li, PAN Jie-xue, DING Guo-lian, HUANG He-feng. 2015, 31(1): 31-35.  DOI: 10.7504/fk2014120107 Abstract ( )   PDF (983KB) ( )   Abstract: Despite many advances in assisted reproduction techniques, implantation rates are still low after in vitro fertilization (IVF) and controlled ovarian stimulation. Successful implantation and pregnancy require a vital embryo and an effective molecular dialogue with a ‘receptive’ endometrium. Endometrial receptivity is the rate-limiting step to the success of IVF. Ovarian stimulation is used in IVF programs to collect multiple oocytes and to produce multiple embryos per cycle of treatment. Although this procedure enables selection of high quality embryos for transfer, ovarian stimulation also results in supraphysiological levels of P and E2. These elevated sex steroid concentrations may impair endometrial receptivity. We try to evaluate the influence of COH on endometrial receptivity from ultrasonic imaging, morphocytology, histomorphology, molecular biology, and gene microarray analysis.

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Luteal support in stimulated ovulation cycle. LIU Dong-e. 2015, 31(1): 35-38.  DOI: 10.7504/fk2014120108 Abstract ( )   PDF (972KB) ( )   Abstract: Stimulating ovulation is a common treatment in the process of infertility treatment, it is also an important part of assisted reproductive technology. Stimulating ovulation may result in luteal phase insufficiency and its effect on endometrium may cause the failure of the embryo implantation, declining of pregnancy rate. Clinical studies suggest luteal support could help pregnancy outcomes in stimulated cycles .There was no generally accepted best luteal support scheme, luteal support drugs mainly include HCG, progesterone and estrogen and GnRH-a. The standards of drug choice, drug delivery methods, dosage form as well as the time of administration are not formed.

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The complications of ovulation induction and its prevention. MA Cai-hong,WANG Yang. 2015, 31(1): 39-43.  DOI: 10.7504/fk2014120109 Abstract ( )   PDF (1028KB) ( )   Abstract: Induction of ovulation often results in multiple follicular development and complications increase signi?cantly, such as multiple pregnancy, ovarian hyperstimulation syndrome (OHSS), ectopic pregnancy, and ovarian tortion. These complications are rare, but can be severe and even life-threatening. It is obvious that the best strategy is prevention of the occurrence rather than dealing with the problem after its occurrence.

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The intervention of TCM for inducting ovulation. SUN Wei. 2015, 31(1): 41-44.  DOI: 10.7504/fk2014120112 Abstract ( )   PDF (703KB) ( )   Abstract: This text outline the research work in the Clinical and experimental about the ovulation with traditional chinese medicine,from the following several aspects:Traditional Chinese medicine dialectical treats、cycle therapy with Traditional Chinese medicine 、monotherapy、Acupuncture treatment、Traditional Chinese medicine external treatment，which indicated its good practical value、developmental prospect and some problems,The paper also put forward to the combination of traditional Chinese Medicine and western medicine on the basis of current problems.

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The selection of method in ovulation induction of the artificial insemination. SHAO Xiao-guang. 2015, 31(1): 43-46.  DOI: 10.7504/fk2014120110 Abstract ( )   PDF (997KB) ( )   Abstract: In the artificial insemination cycle, the ovulation induction must be the ovarian mild stimulation program, to reduce the ovarian hyper-stimulation and the incidence of multiple pregnancies,which is achieved mainly through controlling the start number of follicles in the early follicular phase, and the dominant follicles to ovulate. Clomiphenecitrate and letrozole are the first line program,while low-dose HMG(or FSH) Step-Up is relatively easy to achieve the purpose of control. In the ovulation cycle, detecting the cascade of spontaneous LH peak can get the best pregnancy rates. Thirty-six hours after the HCG trigger may be the best time for completing the artificial insemination surgery.

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The maternal and fetal security after ovarian induction. ZUO Na，WANG Xiu-xia. 2015, 31(1): 46-50.  DOI: 10.7504/fk2014120111 Abstract ( )   PDF (985KB) ( )   Abstract: Ovulation dysfuction includes disturbance of follicular development and oocyte eduction obstacle caused by polycystic ovary syndrome,endometriosis and pelvic inflammatory disease.The application of ovulation induction drugs can solve the problem.The development of assisted reproductive technology depends on COH in order to improve the oocytes retrival rate and pregnancy rate .But under super physiological state of the Gn, estrogen and progesterone and ovulation inducing drugs may affect the health of both mother and children , so people pay more attention on the side effects and security of the ovulation induction drugs.

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The intervention of TCM for inducting ovulation. SUN Wei. 2015, 31(1): 50-52.  DOI: 10.7504/fk2014120112 Abstract ( )   PDF (943KB) ( )   Abstract: This text outlines the research work in the clinics and experiments about the ovulation with traditional Chinese medicine,from the following several aspects:traditional Chinese medicine dialectical treats，cycle therapy with traditional Chinese medicine,monotherapy,acupuncture treatment and traditional Chinese medicine external treatment，which indicated its good practical value,developmental prospect and some problems.The paper also suggests the combination of traditional Chinese medicine and western medicine on the basis of current problems.

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Preliminary study of construction and application of in vivo digitalized three-dimensional navigational modelsfor pelvic lymphadenectomy. DUAN Hui*, CHEN Chun-lin*,LIU Ping*, SU Gui-dong*, GONG Shi-peng*, XU Yi-kai, CHEN Lan*, LI Wei-li, CHEN Rui-ying, TANG Lei, LI Jian-yi, WANG Zhang-lin, LI Peng-fei*. 2015, 31(1): 53-58.  DOI: 10.7504/fk2014120124 Abstract ( )   PDF (1441KB) ( )   Abstract： Objective Construct in vivo digitalized three dimensional navigational models for use in pelvic lymphadenectomy and make a preliminary study of its significance in clinical application. Methods Through the use of the CT angiography DICOM data collection of a patient who was diagnosed at the Department of Gynecology at Southern Medical University's Nanfang Hospital in November 2014 and was scheduled to receive laparoscopic treatment for stage Ⅰb1 cervical cancer, we used digitalized three-dimension reconstruction technology to reconstruct this patients pelvis, pelvic vasculature, psoas major muscle, lymph nodes, obturator nerve and other anatomical structures in order to create a pelvic lymphadenectomy digitalized three-dimensional navigational model. We then used this model as a real-time navigational guide in this patients pelvic lymphadenectomy operation. Results In this study an in vivo pelvic lymphadenectomy digitalized three-dimensional navigational model was successfully created and intra-operative real-time navigational assistance was completed.The abnormal obturator vein belongs to leftillia vein was susessfully avoiding damage during the procedure due to the three dimensional models.During the procedure, through comparison, it was discovered that the reconstructed model and the actual pelvic anatomy of the patient, including the anatomical relationship between the path of pelvic vessels and the surrounding structures such as the pelvis, psoas major and lymph nodes, were identical.The number of lymph nodes 31 excised was slightly more than the number of lymph nodes 24 reconstructed. Conclusions In vivo pelvic lymhpadenectomy digitalized three-dimensional models can successfully assist surgeons to grasp the individual in vivo anatomical characteristics of patients. It offers real-time surgical navigational assistance, preventing injury to important tissues such as blood vessels and increasing operational safety and success rates.

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Clinical study of sequential chemotherapy with paclitaxel plus carboplatin and gemcitabine based medication in reducing recurrence of epithelial ovarian cancer. DU Xue-lian,WANG Cong,LIU Ming,ZHENG Xing,LU Xiang-wei,SHENG Xiu-gui. 2015, 31(1): 59-64.  DOI: 10.7504/fk2014120113 Abstract ( )   Abstract： Objective To study the clinical value of sequential chemotherapy by using paclitaxel plus carboplatin and gemcitabine based medication in preventing drug resistance of epithelial ovarian cancer.Methods Totally 121 patients with operation pathological stageⅢc epithelial ovarian cancer between April 2005 and June 2011 at the Department of Gynecologic Oncology, Shandong Cancer Hospital were analyzed. Two to four cycles of gemcitabine based combination chemotherapy were performed after 4 cycles of paclitaxel-carboplatin in 59 patients (sequential chemotherapy group). Routinely 6 to 8 cycles of paclitaxel-carboplatin were performed in other 62 patients (TC group). Treatment response, recurrence, toxicities, progress-free survival (PFS) and overall survival (OS) were assessed. Results The median follow-up time was 39 mon (4 to 73 mon) and 41 mon (3 to 69 mon), respectively. Similar treatment response and recurrence were found between two groups (P>0.05). Significantly higher PFS was found in sequential chemotherapy group than in TC group (28 vs. 18 mon, P=0.037). Improved 1- and 3-year PFS was observed in sequential chemotherapy group (1-yr: 87.5% vs. 72.4%; 3-yr:35.7% vs.24.1%, P<0.05). Moreover, obviously higher 5-yr OS was found in sequential chemotherapy group (32.2% vs. 18.6%). Three to four levels of nervous system toxicity was significantly lower in sequential chemotherapy group than that in conventional group (3.3% vs. 14.5%，P=0.016).Conclusion Sequential chemotherapy method using 2 to 4 cycles of gemcitabine based combination chemotherapy after 4 cycles of paclitaxel-carboplatin can delay recurrence and obviously improve the 1- and 3 -yr PFS and 5-yr OS with lower nervous system toxicity.

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A multi-center study on the efficacy of immunoprophylaxis for mother-to-infant transmission of hepatitis B virus in multi-centers. ZHANG Lei *, GUI Xi-en*, WANG Bo, HE Xing-ling,ZHANG Ling,ZHU Feng-Yi,LI Li, YE Ping,LI Feng-liang,ZHOU Yun,LIU Xiao-ying. 2015, 31(1): 65-69.  DOI: 10.7504/fk2014120114 Abstract ( )   Abstract： Objective To evaluate the current positive rete of HBsAg among pregnant women and explore more effective interruption measures for mother-to-infant transmission.Methods From 2008 to 2012, pregnant women were screened for HBsAg in multi-centers (Province of Hubei, Shanxi and Guangdong and Xinjiang Uygur Autonomous Region). HBsAg positive mothers before labour and their infants aged 8~12 months in some hospitals among those areas were determined for HBV markers (HBsAg,HBsAb，HBeAg，HBeAb，HBcAb) and some of them also had HBV DNA tests.Results HBsAg positive rate of pregnant women was 6.0% (4924/82214). Infants’ immunoprophylaxis failure rate was 3.1% (42/1371) and it was 8.2% among infants of HBsAg and HBeAg positive mothers. Immunoprophylaxis failure infants were all born to mothers of HBeAg positive and HBV DNA ≥6 log10 copies/mL. Among infants of HBeAg positive mothers, immunoprophylaxis failure rates had no significant difference between mother with hepatitis B immune globulin (HBIG) and without HBIG (8.8% vs. 8.1%, P=0.807).Conclusions These findings demonstrate that pregnant women are still with high HBsAg prevalence (6.0%) in China. HBV mother-to-infant transmission (3.1%) still occurs after active-passive immunization. Pregnant women of HBsAg and HBeAg positive and HBV DNA≥6 log10

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Regulation of miR-145 on p53 in SKOV3 cell of ovarian cancer. LIU Long-hao,WEI Sha-li,YU Qiu-bo,CHEN Yu,LIU Zu-cui,GAOTANG Xin-zi. 2015, 31(1): 70-73.  DOI: 10.7504/fk2014120115 Abstract ( )   Abstract： Objective To explore regulation of miR-145 on p53 in SKOV3 cell of ovarian cancer. Methods With lentivirus transfection technology plasmids of miR-145 were transformed into SKOV3 cell,and by puromycin establish SKOV3 cell strains to express miR-145 steadily. Prove the expressing situation of miR-145 by RT-PCR, relative expression of p53 of SKOV3 after the overexpression of miR-145 was analysed by western-blot from February 2012 to April 2013.Results The expression of protein p53 of SKOV3 increased 1.14 times compared with control group after the overexpression of miR-145 (P<0.05).Conclusion The miR-145 may weaken the malignancy degree by increasing the expression of suppressor gene p53 in SKOV3 cell of ovarian cancer.

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Enhancing effect of Beclin 1 on cytotoxicity of OVCAR3 cells induced by proteasome inhibitors in autophagy-independent manner. LIU Chuan, HU Zhen-hua, LIU Juan-juan, LIU Da-wo, YING Tian-shu, LIN Bei. 2015, 31(1): 74-77.  DOI: 10.7504/fk2014120116 Abstract ( )   Abstract： Objective To investigate the effect and mechanism of Beclin 1 on cytotoxicity of OVCAR3 cells mediated by proteasome inhibitors. Methods OVCAR3 cells were treated with the indicated concentration of MG132 for 24h and cell viability was measured using MTT assay. OVCAR3 cells were transfected with mock or Beclin 1 eukaryotic plasmid, then were treated with the indicated proteasome inhibitors;cell viability was measured using MTT assay. OVCAR3 cells were transfected with shAtg7, the key factor of autophagy at early stage, or cotreated with autophagy inhibitors at late stage, then MTT assay was used to investigate the cytotoxicity of OVCAR3 cells induced by MG132. Results 1,2,5 and 10 μmol/L MG132 resulted in suppression of cell growth of OVCAR3 in concentration-dependent manner;P was 0.023,0.005,0.001 and 0.000. Overexpression of Beclin 1 enhanced the cytotoxicity of OVCAR3 cells mediated by the indicated proteasome inhibitors;P was 0.003,0.008,0.006 and 0.000. Compared with untransfected and control transfected groups, downregulation of autophagy transfected with shAtg7 demonstrated no obvious effect on cytotoxicity of OVCAR3 cells induced by MG132;P was 0.468, 0.667. Cotreated with chloroquine or bafilomycin A1, both demonstrated no obvious effect on cytotoxicity of OVCAR3 cells induced by MG132;P was 0.776 and 0.802.Conclusion Beclin1 enhances the cytotoxicity of OVCAR3 cells mediated by proteasome inhibitors in autophagy-independent manner.

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Relationship between C-reactive protein levels and gestational diabetes mellitus in non-obese pregnant women. LIU Fang,ZHANG Jing-xin,FU Qiang,WEN Jing,GAO Tian-ming. 2015, 31(1): 78-81.  DOI: 10.7504/fk2014120117 Abstract ( )   Abstract： Objective To analyse the relationship between the level of serum C-reactive protein in non-obese pregnant women and gestational diabetes mellitus.To understanding the clinical predictive significance of CRP in GDM. Methods   The body mass index of all the subjects were < 25  before pregnancy.This study included non-obese pregnant women with gestational diabetes mellitus(GDM) and non-obese pregnant women without gestational diabetes mellitus (NGT).The differences in multiple indicators between two groups were compared and the relationship between CRP levels and these indicators was analyzed.Results   The levels of CRP in GDM group were significantly higher than in NGT group(P<0.01).Two groups had significant differences in statistics.The levels of CRP were positively correlated with the HOMA-IR,the weight gain in pregnancy,the fasting glucose,the body weight before prengnacy and the body mass index before pregnancy.Their regression coefficient was 0.377,0.333,0.276,0.278 and 0.300 respectively(all P<0.01).CRP’s linear regression equation y=0.323X1+0.1X2+0.244X3-3.918,r2=0.263 X1HOMA-IR,X2weight gain in pregnancy,X3 the body mass index before pregnancy). Conclusions  The serum CRP level is related to weight gain during pregnancy;it can't confirm that CRP is an independent risk factor for GDM.Controlling the weight gain during pregnancy can prevent the GDM.

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Effect of laparoscopic surgery on cellular immune function of T lymphocyte and recovery after operation in patients with pelvic inflammation mass. HU You-bin*, HE Wen-cong* 2015, 31(1): 82-86.  DOI: 10.7504/fk2014100118 Abstract ( )   Abstract： Objective To examine cellular immune function and postoperative recovery time after receiving laparoscopy operation for pelvic inflammation mass and to investigate the relationship between them. Methods Venous blood samples were collected from patients performed operation for pelvic inflammation mass:32 patients with laparoscopy, the left with abdominal operation. Subgroup of T lymphocyte includeing CD3+，CD4+ and CD8+ was examined by flow cytometer and analyzed with own control;meanwhile,determination of Th1 and Th2 cells was done. Statistics between the two groups, as well as various subgroup Th1, Th2 and, Th1/Th2 ratio differences were analyzed. ELISA method was used for measuring serum IL-18 and IL-10 level and statistical differences between the two groups were analyzed. Postoperative recovery was analyzed,including exhaust time;time of body temperature returning to normal, recovery time of hemogram and length of stay, etc. Results Laparoscopic group T-lymphocyte subsets before and after surgery had no significant difference.One day after laparotomy CD3+, CD4+ and CD8+,compared with the preoperative,were significantly lower after 72h's recovery, but still lower than the preoperative level;comparison of two groups,in the open group CD3+, CD4+ and CD8+ reduced more obvious.In the two groups after two hours,both Th1/Th2 balance to Th2 cells in response to the conversion, Th1 cells, Th1/Th2 ratio, IL-18 decreased(Laparoscopic group: t=2.238, P＜0.05; t=7.15,P＜0.01;t=6.98,P＜0.01; laparotomy group: t=9.45,P＜0.01;t=8.56,P＜0.01;t=10.17,P＜0.01) And Th2 cells, anti-inflammatory cytokines IL-10 increased(Laparoscopic group: t=7.35,P＜0.01;t=8.87,P＜0.01; laparotomy group: t=6.53,P＜0.01;t=7.18,P＜0.01). But the laparoscopic group after 24 hours of the indicators that is restored((t=1.82,P>0.05; t=1.38, P>0.05; t=1.72, P>0.05; t=1.78,P>0.05; t=1.89, P>0.05)), and open group of indicators changes and two hours after a similar, and continued until after 48 hours. Conclusions Pelvic inflammatory disease after cellular immune dysfunction may be involved in post-operative recovery; Pelvic Inflammatory Mass laparoscopic resection group T-cell subsets impact of small, and the Th1/Th2 cell balance and rapid recovery of the body's cellular immune effects of small, faster postoperative recovery.