Abstract:

Abstract： Objective To assess a simple, economic and safe method of chromosome preparation in order to avoid samples mix-up and reduce the cost. Methods One thousand cases of blood samples and one thousand cases of tissue samples including amniotic fluid, chorionic villus and product of conception were taken in Guangxi Maternal and Child Health Care Hospital. All the samples were cultured and harvested by both of traditional and One Tube methods. Results Both methods were showed similar success rates and obtained satisfactory chromosome metaphases. In comparison of the traditional method, the One Tube method results showed that there were not samples mix-up and the average harvesting times were reduced by 1.5 minute for each blood sample and 12 minutes for each tissue sample. The average costs were reduced for each sample, blood 11.1 yuan and tissue 74.4 yuan. There was no incident of sample mix-up and no record of staff injury during the procedure. Conclusion One Tube method is a simple, safe, effective, less cost method of chromosome preparation.

Key words: Nunc tube, one tube method

摘要：

目的 探讨一种在染色体制备过程中避免样本间互相混淆污染、缩短制备时间和降低制备成本的简单方便、经济安全的方法。方法 广西壮族自治区妇幼保健院从2005年起，对1000例血标本、1000例组织培养标本包括羊水、绒毛和流产组织分别使用传统法和一管法进行对照培养和制备染色体。结果 两种方法成功率接近，均获得满意的染色体分裂相；但与传统法相比，运用一管法可以在染色体收获过程中未发生标本混淆的差错事故，并且平均每例血标本收获时间可以节约1.5 min，成本降低11.1 元；每例组织标本可以节约12 min，成本降低74.4 元。结论 一管法也许是一种简便、安全、误差少和成本低值得推广的染色体制备方法。

关键词: Nunc培养管, 一管法