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25 May 2005, Volume 20 Issue 05 Previous Issue    Next Issue
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Expression of Survivin in pediatric acute leukemia and its relationship with expression of P53 and clinical significance Hou Yan,Hu Qun,Liu Aiguo,et al 2005, 20(05): 283-285 .  Abstract ( )   Abstract Objective To investigate the expression of Survivin in pediatric acute leukemia and its relationship with expression of P53 and clinical significance.Methods The expression of Survivin and P53 proteins were detected by immunohistochemical assay in 37 acute leukemia children.The control included 10 children with normal bone marrow.Results Survivin protein expression rate was 35.14% and P53 protein expression rate was 32.43% in 37 AL children.Both of them were significantly higher than that in control group (P<0.05).The expression of Survivin and P53 being strongly related (P<0.05).The expression rate of Survivin was 46.15% in HR ALL and 0% in SR ALL,the difference was statistically significant(P<0.05).Conclusion (1)Survivin protein expression rate is abnormal and is associated with the clinical typing of ALL.It may play an important role in the prognosis of ALL.(2)Expression of Survivin and deactivation of antioncogene P53 might play synergetic roles in the process of genesis of AL.

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Expressions of cyclin D3 and cyclin E in childhood acute leukemia. Liu Zhuang，Wei Hongying，Han Yunli 2005, 20(05): 286-288 .  Abstract ( )   Abstract Objective To investigate the relationship between the expression of cyclin D3，cyclin E and childhood acute leukemia(AL).Methods The expression of cyclin D3 and cyclin E was detected in bone marrow cells from 50 cases of newly diagnosed or relapsing AL，in whom there were 30 cases of acute lymphoblastic leukemia(ALL，including 12 cases of highrisk ALL and 18 cases of standardrisk ALL ) and 20 cases of acute nonlymphocytic leukemia(ANLL)，26 cases of complete remission AL(ALCR) patients and 23 cases of control group(nomalignant disease) using immunohistochemical assay.Results 54% AL cases were positive for cyclin D3，and 46% for cyclin E，both being remarkably higher than that of ALCR group and control group(P<0.01).There was no difference between ALL and ANLL in the expression of cyclin D3 and cyclin E(P>0.05).The positive expression of cyclin D3 was higher in highrisk ALL than that in standardrisk ALL(P<0.01).The expressions of cyclin D3 and cyclin E before chemotherapy were significantly higher than that after remission using chemotherapy(P<0.01).There was significantly positive correlation between the expression of cyclin D3 and cyclin E in AL (r=0.5298，P<0.01).Conclusion In childhood AL，the expression of cyclin D3 and cyclin E is quite high，which is related to the development of AL and might be index of treatment effect.There is positive relation between cyclin D3 and cyclin E.Cyclin D3 might be related to poor prognosis.

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Changes of neuronspecific enolase in children with acute lymphoblastic leukemia during chemotherapy and its significance. Shu Zhirong,Cai Wenxian,Sun Lirong. 2005, 20(05): 289-290 .  Abstract ( )   Abstract Objective To investigate the monitoring factors for the central nervous system from intrathecal injections and highdose methotrexate therapy (HDMTX+IT) given during treatment for acute lymphoblastic leukemia in children.Methods Fortytwo children aged from 5 to 11 years with ALL participated in the study.children had the history of ALL less than one year,while twentytwo children had ALL more than one year.CSF was sampled for analyses of NSE on four occasions.Results No difference was found between the NSE concentration of the children who had ALL more or less than one year.The NSE content in the CSF samples increased from (3.9±0.5) ng/mL before the start of treatment to (6.5±1.1) ng/mL at day 15 and then gradually decreased to (4.3±0.9) ng/mL at day 30 and to (4.0 0.7) ng/mL at day 45.Conclusion NSE is a useful marker for acute brain damage in acute lymphoblastic leukemia children.

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Value of modified method of cerebrospinal fluid preparation and optimized PCR system in diagnosing tuberculous meningitis. Xiang Zhidan,Fang Feng,Shu Sainan 2005, 20(05): 291-293 .  Abstract ( )   Abstract Objective To improve the diagnosing level of tuberculosis meningitis (TBM) by means of modified preparation of cerebrospinal fluid (CSF) and optimized IS6110 PCR system.Methods Establishing the modified templatepreparing method of microwave/ alkaline nonionic detergent/ chloroform and using dimethyl sulphoxide (DMSO) to optimize the PCR system of tubercle bacillus(TB) gene IS6110.Preparing the template from 108 CSF specimens with the microwave/alkaline nonionic detergent/ chloroform method and amplifying IS 6110 gene with DMSO modified system,comparing with the simple chloroform method.ResultsThe modified IS 6110 PCR assay with microwave/alkaline nonionic detergent/chloroform method showed the positive rate of 88%,while the rate of simple chloroform method was 71%.Both false positive rates were 0%. Conclusion The method of preparing the template with the microwave/alkaline nonionic detergent/chloroform method and modifying TB IS 6110 PCR system with 5%DMSO is an effective way to increase the positive rate of TBM diagnosis.

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Psychological intervention study on life quality of children with learning disability by shorttime programmed game. Zhang Xiaolei,Liu Minna,Su Cheng 2005, 20(05): 294-296 .  Abstract ( )   Abstract Objective To evaluate the life quality of the children with learning disability.Methods The quality of life were assessed in children with learning disability pre and postpsychological intervention with shorttime programmed game by Inventory of Subjective Life Quality for children and adolescent(ISLQ) edited by Cheng Zhaohuo.Results The levels of total score,recognition score and emotion score were higher in experimental group than that in control group(P<0.01) after psychological intervention,in which the scores of family life,school life,selfcognition and depression practice are significantly higher than that in control(P<0.01).Conclusion Shorttime programmed group game is an effective psychological intervention method to improve the life quality of children with learning disability.

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Clinical analysis of rhabdomyosarcoma in 8 children . Feng Chen,Tang Suoqin,Huang Dongsheng 2005, 20(05): 297-299 .  Abstract ( )   Abstract Objective To analyze the clinical characteristics of the childhood rhabdomyosarcoma.Methods Clinical data was analyzed in 8 children with rhabdomyosarcoma.Results From Jan,1998 to Oct,2002,eight children with rhabdomyosarcoma had been diagnosed in our department,5 boys and 3 girls,7 months to 11 years old.Primary site was head and neck (n=4),extremities(n=2),kidney (n=1),bile duct(n=1).2,2,1,3 patients were in stage Ⅰ,Ⅱ,Ⅲ,Ⅳ respectively.Pathological data revealed embryonal rhabdomyosarcoma(n=7) and alveolar rhabdomyosarcoma(n=1).The clinical manifestation was mass in primary site or the tumor erodes.All patients had been diagnosed pathologically.The treatment included multimodal therapy with combination of surgery,chemotherapy,and radiation,whose doses were 40 to 50 Gy.Chemotherapy was given to all patients with VDCA、VAC or VadrC.The patients with preStage II have received chemotherapy with protocol of VAC or VadrC for two years.The protocol of VDCA has been used to the patients with Stage Ⅲand Ⅳ for 14 weeks,then the VAC or VadrC has been used until 2 years later.Two year EFS was 37.5%.Conclusion The head and neck are the most common primary site for rhabdomyosarcoma in children,with mainly histological embryonal rhabdomyosarcoma.Early diagnosis and combination therapy including surgery,chemotherapy,and radiation are key for those with rhabdomyosarcoma to get long EFS.

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Multislice spiral computed tomography in detecting congenital heart diseases in children Li Jianglin,Li Yufen,Zhang Zhiwei 2005, 20(05): 300-302 .  Abstract ( )   Abstract Objective To evaluate the effectiveness of multislice computed tomography (MSCT) in diagnosis of congenital heart diseases in children.Methods 86 patients were involved.69 had been performed operations.The preoperative results of transthoracic two dimensional echocardiography (TTE),MSCT and angiocardiography were compared with those of surgery.Results Of the totally 129 malformations,TTE had correctly revealed 116 (90%),MSCT 112(86.8%),and both combined had correct diagnosis for 127(98.4%).Angiocardiography in 12 patients had not provided further information. Conclusion MSCT is a valuable additional diagnosis tool in congenital heart diseases,especially when combined with TTE.They might take place some of the angiocardiography before surgery.

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The research of fluorescence quantitative polymerase chain reaction (FQ-PCR) method to test children virus encephalitis. Gao Wenjuan,Yan Shijun,Wang Yunxia 2005, 20(05): 302-304 .  Abstract ( )   Abstract Objective To discuss the value of using FQ-PCR to check pathogen in children viral encephalitis.Methods From Janurary,2002 to October,2004,PCR was used to select the children of viral encephalitis and check the six kinds of common virus which cause viral encephalitis and then we used FQ-PCR to check the children with HSV(+),CV(+)EV(+) while checking the copied number of HSVE-DNA before,during and after treatment.Results It was 100% that FQ-PCR checked the sample of PCR (+).The number of HSVDNA was different at three time points. The copied number of virus DNA was consistent with clinical symptom.Conclusion FQ-PCR is one of the effective methods to diagnose the children's viral encephalitis.Observing the number of HSVDNA is beneficial for instructing,treating and estimating the development and future disease.