Abstract:

Abstract：Objective　To observe the levels of Th17 cells，CD4+CD25+ regulatory T cells in the asthmatic children and relationship between the two types of cells and children’s condition. Methods　Flow cytometry was used to detect the percentages of the Th17 cells and CD4+CD25+ regulatory T cells in the peripheral blood of acute asthma children （asthma group， n = 60），alleviated period asthma children （n = 30） and healthy children （healthy control group， n = 30）.Acute asthma children were divided into 3 groups： mild， moderate and severe asthmatic patients. Results　Compared with the healthy control group （1.02%±0.28%）and alleviated period asthma children （1.65%±0.38%）， the numbers of CD4+ cells （Th17） expressing IL-17 in peripheral blood of acute asthma（2.24%±1.02%） were increased， and the differences were statistically significant （P < 0.05）. The levels of CD4 + CD25+T cells in peripheral blood of acute asthma and alleviated period asthma children （5.37%±0.80%； 6.05%±0.87%） were significantly lower than those of healthy children（7.11%±0.89%） （P < 0.05）. Th17 cells were positively correlated with the course of childhood asthma（r =  0.649，P < 0.05）.CD4+CD25+ regulatory T cells were negatively correlated with the course of childhood asthma （r =-0.599， P < 0.05）. Conclusion　The immunization response of Th17 cells in peripheral blood of asthmatic children is strengthened，but the immune function of CD4+CD25+regulatory T cells is decresed. The severity of asthma is closely related to the inbalance of Th71/Treg cellular immunity.

Key words: asthma, Th17 cells, CD4+CD25+regulatory T cells, children, T cell subsets

摘要：

摘要：目的　探讨支气管哮喘患儿外周血中辅助T细胞（Th）17细胞和CD4+ CD25+调节性T细胞（Treg）的变化与儿童哮喘病情的相关性。方法　收集2009年月5月至2010年4月于郑州大学第一附属医院就诊的患儿，均为首次确诊哮喘或规范吸入激素停用 > 3个月后复发及近1个月内无明显感染者。采用流式细胞仪测定患儿外周血中Th17细胞及CD4+CD25+Treg比例的变化。结果　Th17细胞在哮喘急性期组（2.24%±1.02%）较哮喘缓解期组（1.65%±0.38%）及健康儿童组（1.02%±0.28%）均显著增高（P < 0.05），哮喘缓解期组（1.65%±0.38%）和健康儿童组（1.02%±0.28%）无明显差别，CD4+CD25+Treg细胞比例在3组儿童间差异均有统计学意义（F = 45.604，P < 0.05），与健康儿童组（7.11%±0.89%）相比，哮喘缓解期组（6.05%±0.87%）和哮喘急性期组（5.37%±0.80%）的比例明显下降，而哮喘急性期组较健康儿童组下降。哮喘急性期组轻、中、重度3组之间差异同样有统计学意义。Th17细胞与哮喘患儿病情呈正相关（r = 0.649，P < 0.05），而CD4+CD25+Treg细胞与病情呈负相关（r = -0.599，P < 0.05）。结论　急性期哮喘患儿Th17免疫应答增强，急性期患儿、缓解期CD4+CD25+Treg细胞免疫功能下降，哮喘的严重程度与外周血中的Th17/Treg细胞免疫失衡密切相关。

关键词: 哮喘, Th17细胞, 调节性T细胞 , 儿童, T细胞亚群