Abstract:

Objective　To explore the mechanisms of visfatin in fetal growth. Methods　From Dec.2008 to Dec.2009，the cord blood plasmaconcentrations of visfatin in 90 term neonates at birth in Wuxi Maternal and Child Health Hospital，Wuxi No.3 People’s Hospital and Wuxi Xishan Hospital was measured by Elisa and its mRNA expression by quantitative real-time PCR in placenta.The correlation between birth weight ，cord blood visfatin，and mRNA expression was analyzed. Results　Cord blood concentration of visfatin in IUGR were significantly higher than that of fetal macrosomia and healthy term infants（P < 0.05），while there was no difference between fetal macrosomia and healthy term infants（P > 0.05）.There was no difference in visfatin mRNA expression in placenta between the three subgroups. Regression analysis revealed that cord blood visfatin levels were inversely（r = -0.416，P < 0.05）correlated with birth weight，while visfatin mRNA expression was correlated with neither birth weight nor cord blood visfatin（P > 0.05）. Conclusion　Concentrations of cord blood visfatin are significantly higher in the IUGR than in the fetal macrosomia and healthy term infants，although no differences in its mRNA expression in placenta are observed.

Key words: intrauterine growth retardation （IUGR）, cord blood, visfatin, placenta

摘要：

目的　探讨内脂素调节胎儿生长发育的可能作用机制。 方法　选择2008年12月至2009年12月在南京医科大学附属无锡妇幼保健院、无锡市第三人民医院及无锡市锡山人民医院产科足月出生的宫内发育迟缓（IUGR）患儿、巨大儿及正常足月儿各30例，采用ELISA法测定脐血内脂素浓度，实时聚合酶链反应（Real-time PCR）法检测胎盘内脂素mRNA相对含量，分析脐血内脂素浓度与胎盘内脂素mRNA相对含量间的关系及其两者与出生体重的关系。 结果　IUGR患儿脐血内脂素浓度高于巨大儿及正常足月儿，差异有统计学意义（P < 0.05）；巨大儿与正常足月儿之间脐血内脂素浓度差异无统计学意义（P > 0.05）。脐血内脂素浓度与出生体重呈负相关性（r = -0.416，P < 0.05）。3组间胎盘内脂素mRNA表达差异无统计学意义（P < 0.05），胎盘内脂素mRNA表达与出生体重、脐血内脂素浓度均无相关性（P > 0.05）。 结论　新生儿期内脂素与IUGR相关，可能导致出生后体内脂肪组织快速增加。

关键词: 宫内发育迟缓, 脐血, 内脂素, 胎盘