The activation of nuclear factorKappa B in kidney tissue in children with primary nephrotic syndrome and its relationship with pathological changes and urine protein excretion.

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The activation of nuclear factorKappa B in kidney tissue in children with primary nephrotic syndrome and its relationship with pathological changes and urine protein excretion.

Zhao Hongyang,Sun Ruopeng,Zhen Junhui et al.

Department of Pediatrics,Qilu Hospital of Shandong University,Jinan 250012,China

Received:2004-12-12 Revised:2005-03-10 Online:2005-06-25 Published:2005-06-25
Contact: Zhao Hongyang

原发性肾病综合征患儿肾组织核因子-κB活化水平与病理类型及尿蛋白关系

赵红洋¹;孙若鹏¹;甄军晖²;董俊华¹

1山东大学齐鲁医院小儿内科(250012);2山东大学齐鲁医院病理科(250012)

通讯作者: 赵红洋

Abstract

Abstract: Abstract Objective To study activation of transcription factor NFKappa B(NF-κB) and its role in regulating the expression of inflammatory mediators involved in pathogenesis of primary nephrotic syndrome (PNS).Methods To explore the role of NF-κB in the pathogenesis of PNS in children,and its significance for pathological changes as well as 24hour urine protein excretion,we examined NF-κB activity in glomeruli and tubular tissues by twostep immunohistochemical staining from the kidney biopsy specimen of 51 children with PNS,and from 5 patients after renal operation.Results We found that NF-κB activity significantly increased both in MCD and NMCD children compared with the controls,and the activation of NF-κB in MCD was different from that of NMCD;there was also statistical difference of NF-κB activity between mild,moderate,severe MsPGN and the controls Additionally,positive correlation was observed between NF-κB activity of glomeruli and renal tubules and 24hour urine protein excretion.Conclusion These results suggested that the upregulated activation of NF-κB in glomerular and tubular tissues of children with PNS is related to severity,pathological changes and urine protein excretion,and involved in the pathogenesis of PNS.

Key words: Children , Primary nephrotic syndrome, Pathology

摘要： 目的探讨原发性肾病综合征(PNS)患儿肾组织核因子-κB(NF-κB)活化水平及其与肾组织病理类型、24h尿蛋白定量的关系。方法 2002年2月至2004年9月，收集山东大学齐鲁医院小儿内科51例PNS患儿活检肾组织及5例肾脏手术切除的正常肾组织。采用免疫组织化学染色法及多媒体彩色病理图文分析系统MPIAS-500，观察PNS患儿各病理类型肾组织及正常肾组织NF-κB的活化水平，同期收集PNS患儿24h尿蛋白定量。结果微小病变型(MCD)、非微小病变型(NMCD)PNS患儿肾小球及肾小管中NF-κB活化水平均较对照组显著增强；NMCD患儿肾小球及肾小管中NF-κB的表达水平显著高于MCD患儿；20例系膜增殖性肾小球肾炎(MsPGN)患儿肾组织NF-κB活化水平随系膜增殖程度加重而增高；PNS患儿肾小球及肾小管NF-κB表达水平与24h尿蛋白定量呈显著正相关。结论PNS患儿肾小球及肾小管中NF-κB活化水平上调，且与组织病理类型、尿蛋白量相关，NF-κB的异常活化在PNS发病中起重要作用。

Abstract Objective To study activation of transcription factor NFKappa B(NF-κB) and its role in regulating the expression of inflammatory mediators involved in pathogenesis of primary nephrotic syndrome (PNS).Methods To explore the role of NF-κB in the pathogenesis of PNS in children,and its significance for pathological changes as well as 24hour urine protein excretion,we examined NF-κB activity in glomeruli and tubular tissues by twostep immunohistochemical staining from the kidney biopsy specimen of 51 children with PNS,and from 5 patients after renal operation.Results We found that NF-κB activity significantly increased both in MCD and NMCD children compared with the controls,and the activation of NF-κB in MCD was different from that of NMCD;there was also statistical difference of NF-κB activity between mild,moderate,severe MsPGN and the controls Additionally,positive correlation was observed between NF-κB activity of glomeruli and renal tubules and 24hour urine protein excretion.Conclusion These results suggested that the upregulated activation of NF-κB in glomerular and tubular tissues of children with PNS is related to severity,pathological changes and urine protein excretion,and involved in the pathogenesis of PNS.
Key words Nuclear factor kappa B;Primary nephrotic syndrome;Pathology;Children

关键词: 核因子κB, 肾病综合征, 病理学, 儿童

Zhao Hongyang,Sun Ruopeng,Zhen Junhui et al.. The activation of nuclear factorKappa B in kidney tissue in children with primary nephrotic syndrome and its relationship with pathological changes and urine protein excretion.[J]. .

赵红洋,孙若鹏,甄军晖,董俊华. 原发性肾病综合征患儿肾组织核因子-κB活化水平与病理类型及尿蛋白关系[J]. 中国实用儿科杂志.

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[3]	WANG Hui, LIANG Ying. Treatment for renal damage in children with IgA vasculitis [J]. Chinese Journal of Practical Pediatrics, 2022, 37(1): 29-33.
[4]	WANG Fang. Childhood IgA vasculitis with nephritis and IgA nephropathy [J]. Chinese Journal of Practical Pediatrics, 2022, 37(1): 33-36.
[5]	CUI Fei-fei，CHEN Chao，LIU Jing，et al. Distribution of central sleep apnea in children with obstructive sleep apnea hypopnea syndrome [J]. CJPP, 2021, 36(6): 459-462.
[6]	XU Jing*，WANG Xiao-qiu，LI Wen-xiu，et al. A cross-sectional survey and analysis of childhood allergic diseases in two communities of Yangpu District，Shanghai [J]. CJPP, 2020, 35(9): 722-726.
[7]	DU Yu-hui*，LI Jing，LIU Cheng-jun. Relationship between glycaemic variability and outcomes in critically ill children at different age [J]. CJPP, 2020, 35(6): 471-477.
[8]	WANG Xian-zhu，LI Jiu-jun. Risk factors of purulent meningitis complicated with subdural effusion in infants and young children [J]. CJPP, 2019, 34(5): 410-413.
[9]	GUO Jiang-bo，LUO You-you. Macroamylasemia in one child and the literature review [J]. CJPP, 2019, 34(4): 315-316.
[10]	SHEN Kun-ling*，SHANG Yun-xiao，ZHANG He，et al. A multicenter，randomised，controlled clinical study on efficacy and safety of recombinant human interferon α2b spray（P.putida） in treatment of acute upper respiratory tract infection in children [J]. CJPP, 2019, 34(12): 1010-1016.
[11]	GENG Ling-ling*，MIAO Feng，ZHOU Yang，et al. Efficacy and safety of tocilizumab on refractory systemic onset juvenile idiopathic arthritis：observation on 16 cases [J]. CJPP, 2019, 34(1): 37-40.
[12]	HUANG Zong-yao*，LUO Rong，ZHANG Chuan，et al. Efficacy and safety of carnitine for epileptic children using valproic acid：An evidence-based evaluation [J]. CJPP, 2018, 33(8): 626-632.
[13]	YANG Hai-ping，LI Qiu. Obesity-related glomerulopathy in children [J]. CJPP, 2018, 33(2): 102-105.
[14]	LUAN Xiao-fei，JIANG Hong. Correlation analysis between different levels of proteinuria and renal pathological changes in children with IgA nephropathy [J]. CJPP, 2018, 33(2): 127-130.
[15]	YUAN Jing*，ZHU Ming，ZHAO Xu-sheng，et al. Analysis of the serum 25-hydroxyvitamin D level in 2416 children aged from 0 to 14 years old [J]. CJPP, 2018, 33(2): 145-147.