中国实用儿科杂志

中国实用儿科杂志 ›› 2012, Vol. 27 ›› Issue (10): 774-776.

• 论著 • 上一篇    下一篇

儿童难治性癫痫外周血多药耐药
相关蛋白1表达研究

岳    璇1，刘晓鸣2，袁宝强1，李    瑞2   

  1. 1.徐州医学院附属医院儿科，江苏徐州    221000；2 .徐州市儿童医院，江苏徐州    221000
  • 出版日期:2012-10-06 发布日期:2012-09-24

Study of MRP1 expression in peripheral blood of children with refractory epilepsy.  

  1.  YUE Xuan*，LIU Xiao-ming，YUAN Bao-qiang，LI Rui.Department of Pediatrics，the Affiliated Hospital of Xuzhou Medical College，Xuzhou 221000，China.
  • Online:2012-10-06 Published:2012-09-24

摘要：

目的    研究儿童难治性癫痫外周血多药耐药相关蛋白1（MRP1）的表达情况。方法    2010年11月至2011年10月徐州市儿童医院门诊及住院收治的癫痫患儿60例，分为难治性癫痫组和药物控制良好组，各30例；选取同期来院体检的健康儿童30名为对照组。采用逆转录酶聚合酶链式反应（RT-PCR）和蛋白免疫印迹（Western blot）方法测定3组外周血MRP1 mRNA及蛋白含量。结果    难治性癫痫组MRP1表达明显高于药物控制良好癫痫组及对照组，差异均有统计学意义（P＜0.05）；药物控制良好癫痫组与对照组比较差异无统计学意义（P＞0.05）。结论    难治性癫痫患儿外周血MRP1表达增强。

关键词: 难治性癫痫, 多药耐药相关蛋白1, 儿童, 外周血

Abstract:

Objective    To investigate the expression of multidrug resistant-associated protein 1 in the peripheral blood of children with refracotory epilepsy. Methods    The expression of MRP1 was detected by RT-PCR and Western blot in children with intractable epilepsy，epilepsy controlled by anti-epileptic drugs （AEDs） and healthy children.Results    The two methods showed that MRP1 expression in intractable epilepsy was higher than that in epilepsy controlled by anti-epileptic drugs （AEDs） and healthy children（P＜0.05）， and there was no difference between epilepsy controlled by anti-epileptic drugs （AEDs） and healthy children.Conclusion    MRP1 overexpresses in peripheral blood of children with refractory epilepsy.

Key words: refactory epilepsy, multidrug resistant-associated protein 1, children, peripheral blood