中国实用儿科杂志

中国实用儿科杂志

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糖皮质激素受体α和受体β与特发性血小板减少性紫癜糖皮质激素抵抗的关系

王缨，李长钢，石红松，唐沂，王国兵   

  1. 深圳市儿童医院血液内科，广东深圳518026
  • 收稿日期:2006-07-21 修回日期:2006-10-01 出版日期:2007-02-06 发布日期:2007-02-06

A study on the relationship between α and βisoform of glucocorticoid receptors and glucocorticoid in resistant idiopathic thrombocytopenia purpura.

WANG Ying,LI Changgang,SHI Hongsong,et al.   

  1. Department of Hematology,Shenzhen Children's Hospital.Shenzhen 518026,China
  • Received:2006-07-21 Revised:2006-10-01 Online:2007-02-06 Published:2007-02-06

摘要： 目的探讨糖皮质激素受体α(GRα)和糖皮质激素受体β(GRβ)在原发性血小板减少性紫癜(ITP)糖皮质激素抵抗中的分子生物学机制。 方法选择200301—200512在深圳市儿童医院血液内科住院的ITP患儿30例，将患儿分为2组：激素敏感组(18例)和激素抵抗组(12例)，另选10例正常儿童作对照。应用RTPCR法检测激素敏感组、激素抵抗组ITP患儿及10例正常儿童外周血单个核细胞GRα和GRβ mRNA的表达水平，以探讨GRα和GRβ与ITP糖皮质激素抵抗的关系。 结果(1)对照组、激素敏感组和激素抵抗组3组之间GRα mRNA的表达［分别为(0.75±0.17)、(0.70±0.15)、(0.73±0.16)］差异无显著性，P>0.05；(2)对照组和激素敏感组的GRβ mRNA［分别为(0.13±0.03)、(0.15±0.04)］差异亦无显著性，P>0.05，但激素抵抗组的GRβ mRNA水平［为(0.39±0.12)］显著高于对照组和激素敏感组，P<0.01,差异具有统计学意义。 结论ITP患儿糖皮质激素抵抗可能与GRβ mRNA的表达亢进有关。

关键词: 紫癜, 血小板减少性, 特发性, 受体, 糖皮质激素, 激素抵抗

Abstract: AbstractObjectiveTo identify the relationship between the expression of α and βisoforms of glucocorticoid receptors(GR)and response to glucocorticoid in the patients with idiopathic thrombocytopenia purpura (ITP). MethodsSemiquantitative RTPCR was used to detect the expression of GRα,β in PBMC among 30 patients with ITP and 10 normal controls. Results(1)The expression of GRαmRNA in PBMC showed among the groups.(2)The expression of GRβmRNA in the resistant group of ITP was significantly higher than that in both the sensitive group and control group (P<0.01). ConclusionThe expression of GRβmRNA may play a great role in resisting glucocorticoid resistance in the patients with ITP.

Key words: Receptors, Glucocorticoid, Resistance , Thrombocytopenia, Idiopathic