Abstract:

Objective　To detect the inhibitory effect of emodin-8-O-β-D-glucopyranoside on sialidase activity and virulence genes expression of Porphyromonas gingivalis（P. gingivalis）. Methods    Different concentrations of emodin-8-O-β-D-glucopyranoside（0.2，0.5，2，5，10 mg/mL）were added in P. gingivalis W83 cultural medium（experiment groups）. The control group consisted of the untreated P. gingivalis W83. The inhibitory effect of emodin-8-O-β-D-glucopyranoside on P. gingivalis sialidase was verified by using fluorometric assay. At a concentration of emodin-8-O-β-D-glucopyranoside （5 mg/mL），the effect on virulence genes expression in P. gingivalis （fimA，fimR，fimS，kgp，rgpA and rgpB） was determined by Real-time PCR. Results    When the concentrations were 0.2，0.5，2，5 and 10 mg/mL，the inhibition rates of sialidase activity were 11.4%、32.23%、40.21%、73.54% and 84.31%，respectively. Compared with control group，the expressions of fimA，fimR，fimS，kgp，rgpA and rgpB in experiment groups were decreased significantly （P < 0.05）. Conclusion    Emodin-8-O-β-D-glucopyranoside exhibits effective inhibition on sialidase activities in P. gingivalis，and decreases the expression of gene virulence，which may become a new drug in the prevention and treatment of periodontitis.

Key words: Porphyromonas gingivalis；sialidase；emodin-8-O-&beta, -D-glucopyranoside

摘要：

目的    探讨大黄素-8-O-β-D-吡喃葡萄糖苷对牙龈卟啉单胞菌（P. gingivalis）唾液酸酶活性及其毒力基因表达的影响。方法    使用不同质量浓度的大黄素-8-O-β-D-吡喃葡萄糖苷（0.2、0.5、2、5、10 mg/mL）处理P. gingivalis W83（实验组），用未加药物的P. gingivalis W83作对照（对照组），采用荧光法检测大黄素-8-O-β-D-吡喃葡萄糖苷对P. gingivalis唾液酸酶活性的作用。5 mg/mL大黄素-8-O-β-D-吡喃葡萄糖苷作用于P. gingivalis W83，Real-time PCR法检测毒力基因fimA、fimR、fimS、kgp、rgpA和rgpB的表达情况。结果    大黄素-8-O-β-D-吡喃葡萄糖苷对P. gingivalis唾液酸酶活性产生了抑制作用，当其质量浓度为0.2、0.5、2、5、10 mg/mL时，对唾液酸酶活性的抑制率分别为11.4%、32.23%、40.21%、73.54%、84.31%。与对照组比较，实验组（5 mg/mL大黄素-8-O-β-D-吡喃葡萄糖苷处理）的fimA、fimR、fimS、kgp、rgpA和rgpB基因表达均下降，差异均有统计学意义（均P < 0.05）。结论    大黄素-8-O-β-D-吡喃葡萄糖苷可有效抑制P. gingivalis唾液酸酶活性，其抑制作用会降低细菌毒力基因表达，有望成为预防及治疗牙周炎的新型药物。

关键词: 牙龈卟啉单胞菌；唾液酸酶；大黄素-8-O-&beta, -D-吡喃葡萄糖苷