Abstract:

Objective    To investigate the expression of miR-590 in the tissues of patients with oral lichen planus and oral squamous carcinoma and study its role in the carcinogenesis of oral mucosal cells. Methods    Twenty female and twelve male OLP patients（OLP group）and seventeen female and eleven male OSCC patients（OSCC group）were selected，who were pathologically diagnosed in Jinzhou Stomatological Hospital from March 2014 to December 2015. Twenty healthy matched volunteers served as control group. The expressions of miR-590 in oral tissues of the three groups were examined by realtime PCR. The difference among the three groups was statistically analyzed. Results    The relative expressions of miR-590 were significantly higher in OLP（1.96 ± 0.52）and OSCC patients（2.75 ± 0.78）than those in the healthy controls（0.77 ± 0.34）（P < 0.05）. There were significant differences in the expression of miR-590 between the OLP group and OSCC group（P < 0.05）. Conclusion    The expressions of miR-590 are higher in OLP and OSCC patients than those in normal controls；miR-590 may play a role in the carcinogenesis of oral mucosal cells.

Key words: microRNA, miR-590, oral lichen planus, oral squamous carcinoma

摘要：

目的    研究口腔扁平苔藓（oral lichen planus，OLP）及口腔鳞癌（oral squamous carcinoma，OSCC）患者口腔黏膜组织中微小RNA-590（miR-590）的表达，探讨其在口腔黏膜细胞癌变中的作用及意义。方法    选择2014年3月至2015年12月间锦州市口腔医院收治且经病理学确诊的OLP患者32例（OLP组）和OSCC患者28例（OSCC组），所有OLP患者均为初诊且经病理学确诊取材，OSCC患者均为术中病理确诊后开始取材。另选择20名健康志愿者作为对照组。采用实时荧光定量PCR技术检测3组受试者口腔黏膜组织中miR-590的表达水平并进行统计学分析。结果    OSCC组患者的miR-590相对表达水平（2.75 ± 0.78）最高，OLP组的相对表达水平（1.96 ± 0.52）次之，均显著高于对照组（0.77 ± 0.34），差异有统计学意义（P < 0.05）。OSCC组与OLP组的miR-590表达差异有统计学意义（P < 0.05）。结论    与正常对照比较，miR-590的表达在OSCC和OLP组中显著升高，miR-590可能参与了口腔黏膜细胞癌变过程。

关键词: 微小RNA, miR-590, 口腔扁平苔藓, 口腔鳞癌