Abstract:

Objective    To invesigate the combined effect of bioactive hyaluronic acid（B-HA） with minocycline hydrochloride on the antibacterial function of Porphyromonas gingivalis（P.gingivalis），to provide a therory to the clinical of periodontal medicine. Methods    Agar dilution method with medicine to measure the survive rate of P.gingivalis，divided into four groups，B-HA separately，minocycline hydrochloride separately，B-HA combined minocycline hydrochloride，control group accordingly. Quantitated the ability of P.gingivalis is growth by counting the number of the visible bacterial colony，and computed the survival rate of P.gingivalis. Results    Five different density of B-HA combined the minocycline hydrochloride in a density of 0.05 μg/L ，the result showed as the increase of B-HA，the survival rate is decrease gradually（P < 0.05），the survival rate is 82.57%，71.56% seperately when the density is 35 mg/L and 45 mg/L respectively. The survival rate is 50.46% when the density is 55 mg/L. Compared to the B-HA alone，it has improved at 22.93%（P < 0.05）. Conclusion    Our research show that the combination between B-HA and minocycline hydrochloride has signifiant effect on the anti-bacterial of P.gingivalis.

Key words: hyaluronic acid, HA；Porphyromonas gingivalis；minocycline hydrochloride；combined antibacterial effect；agar dilution method

摘要：

目的    探究生物活性透明质酸（B-HA）联合盐酸米诺环素对牙龈卟啉单胞菌（P.gingivalis）的抑菌效果，为B-HA联合盐酸米诺环素在牙周病治疗领域的应用提供理论依据。方法    采用细菌平板计数法定量检测实验药物对P.gingivalis的抑菌作用，共分为4组，分别为空白对照组、单纯B-HA组、单纯盐酸米诺环素组以及盐酸米诺环素联合B-HA组。通过计数平板中可见菌落的数量，计算P.gingivalis存活率。结果    将浓度为0.05 μg/L的盐酸米诺环素与不同浓度的B-HA相联合，与单纯盐酸米诺环素相比，随着B-HA浓度逐渐升高，细菌的存活率逐渐降低，差异有统计学意义（P < 0.05），当B-HA浓度为35 mg/L和45 mg/L时，细菌存活率分别为82.57%和71.56%（P < 0.05）。当B-HA浓度为55 mg/L时，仅50.46%细菌存活，与单纯B-HA相比，抑菌能力提升了22.93%（P < 0.05）。结论    B-HA联合盐酸米诺环素对P.gingivalis具有显著的协同抑菌作用。

关键词: 透明质酸, 牙龈卟啉单胞菌, 米诺环素, 联合抑菌, 琼脂平板稀释法