Abstract:

Objective    To observe the demineralization of different selenium level on rat tooth and investigate the relationship between selenium concentration and caries susceptibility. Methods    From August to October of 2013，12 pregnant Wistar rats were divided randomly into four groups according to body weight after pregnancy. They were respectively fed with 0 mg/kg，0.015 mg/kg，0.15 mg/kg and 1.5 mg/kg selenium diet until 21 days after parturition. Then the offspring were fed with selenium diet as well as the female rats. The filial rats were killed at postnatal day 44. Their mandibular posterior teeth were cut and they were dipped into an artificial caries solution. Then their demineralization was examined by enhanced visual methods，DIAGNOdent and confocal laser scanning microscope（CLSM）. Results    There was significant difference among four groups in degree of demineralization（P＜0.05）. All the three methods showed the degree of demineralization became more serious with an increasing selenium concentration，but the demineralization of low selenium group was obviously lower than that of control group. Conclusion    Different selenium intake can have an influence on caries susceptibility during developmental stage. High level of selenium may imply an increased risk of caries while low level of selenium may decrease caries incidence.

Key words: selenium, demineralization, caries susceptibility

摘要：

目的    观察不同硒水平作用的大鼠牙齿脱矿情况，探讨硒与龋易感性的关系。方法    2013年8月至10月于吉林大学口腔医学院将12只妊娠Wistar大鼠按体重随机分为4组，自受孕之日开始分别喂食无硒（对照组）、低硒（含硒量0.015 mg/kg）、中硒（含硒量0.15 mg/kg）、高硒（含硒量1.5 mg/kg）饲料，喂至母鼠分娩后21 d，给断乳仔鼠继续喂食原饲料。处死44日龄仔鼠，取其上下颌后牙经人工致龋液处理，用增强肉眼光学检查、激光龋齿诊断仪DIAGNOdent、激光共聚焦扫描显微镜（CLSM）检测不同硒水平作用下大鼠牙齿脱矿程度。结果    4组大鼠牙齿脱矿程度差异均有统计学意义（均P＜0.05）。增强肉眼光学检查、激光龋齿诊断仪及CLSM 等3种不同方法检测均显示随硒增高大鼠牙齿脱矿程度加重，但低硒组脱矿程度明显低于对照组，差异有统计学意义（P＜0.05）。结论    发育时期摄入硒能影响牙的龋易感性，高水平硒使大鼠牙齿更易于龋坏，低水平硒可降低龋易感性。

关键词: 硒, 脱矿, 龋易感性