Abstract:

Abstract：Objective To study a culture model of embryonic palatal mesenchymal（EPM）cells in BALB/c inbred mice and discuss the biological characteristics of the EPM cells in vitro. Methods The experiment was made in the Institute of Medical Parasitology，Shanxi Medical University，from November 2012 to July 2013. The mice EPM cells were harvested from a female with day 15 of gestation by dissection and sequential digestion with 0.25% trypsin. The isolated cells were cultured in DMEM/F-12 medium and identified by immunohistochemical method. The cells were checked by immunohistochemistry for in vitro proliferation. Results EPM cells were fibroblast-like cells with single swirling arrangement，and they were disordered with large nuclei and much mitotic. Immunohistochemistry results showed negative for cytokeratin markers，vimentin labeled as positive. EPM cells showed exponential growth，a strong proliferation. Conclusion The culture model of EPM cells of BALB/c mice is successfully created in vitro and EPM cells in this culture system can keep their basic biological features.

Key words: BALB/c inbred mice, cell culture, embryonic palatal mesenchymal cell

摘要：

目的 建立BALB/c近交系小鼠腭胚突间充质（EPM）细胞体外培养模型并研究其生物学特性。方法 本研究于2012年11月至2013年7月在山西医科大学医学寄生虫研究所进行。采用机械分离及胰蛋白酶消化法，简便、准确、快速地获得高成活率的BALB/c近交系小鼠EPM细胞，采用免疫组化法鉴定细胞，并进行细胞增殖和形态学观察。结果 EPM细胞为成纤维细胞样细胞，呈梭型，单层漩涡状排列，排列无序，细胞核大，核分裂像较多。免疫组化染色显示，角蛋白标记为阴性，波形蛋白标记为阳性。EPM细胞呈指数生长，有较强的增殖能力。结论 建立的BALB/c近交系小鼠EPM细胞体外培养技术模型可较好地保持EPM细胞的基本生物学特性。

关键词: BALB/c近交系小鼠, 细胞培养, 腭胚突间充质细胞