Abstract:

Abstract：Objective To detect the influence of high glucose circumstance on expression of correlation factors including TRAIL， OPG and OPGL and proliferating cycle of human osteoblast （MG63） in order to make sure whether high concentration of blood glucose can have relationship with physiological functions of human osteoblasts. Then the pathogenesis of diabetes mellitus with chronic periodontitis can be explored which may provide theoretical basis for clinical orthodontic treatment. Methods Firstly， MG63 cells were put into medium of different concentrations （5.5， 8， 11， 20， 30mmol/L） for 24 hours. Then several measurements were carried out；MTT colorimetric measurement was used to detect proliferation；flow cytometry was used to detect cell cycle； reverse transcription polymerase chain reaction （RT-PCR） was used to detect the expression of TRAIL， OPG， OPGL and mRNA. All the data were analyzed by SPSS 16 software. Results Low concentration of glucose had no significant influence on the proliferation of MG63 cells and other biological activity； with the concentration of glucose increasing， the proliferation of MG63 cells was inhibited （the percentage of G1 phase cells increased；the proportion of S phase unchanged；the percentage of G2 phase cells decreased）；with the increase of glucose concentration， expression of TRAIL and OPGL mRNA of MG63 cells showed an increasing trend （P < 0.05）， but OPG showed opposite trend. Conclusion Circumstance of high glucose can inhibit MG63 proliferation and lead to the increasing expression of TRAIL and OPGL and the decreasing expression of OPG of human osteoblast.

Key words: high glucose, MG63 cell, cell cycle, tumor necrosis factor-related apoptosis inducing ligand

摘要：

目的 研究高糖环境对具有人类成骨细胞表型特征的MG63细胞增殖周期以及相关因子（TRAIL、OPG、OPGL）表达的影响，以期确定高浓度的葡萄糖是否影响人类成骨细胞的生理功能，进一步探讨糖尿病伴慢性牙周炎的发病机制，并为临床正畸治疗提供一定的理论依据。方法 本实验于2012年2—5月在药物基因与蛋白筛选国家工程实验室（东北师范大学）完成。采用含不同浓度葡萄糖（5.5、8、11、20、30 mmol/L） 的培养基分别培养MG63细胞24 h后，四甲基偶氮唑盐（MTT）比色法测定细胞增殖，流式细胞仪检测细胞周期，逆转录聚合酶链式反应（RT-PCR）检测细胞中TRAIL、OPG、OPGL mRNA的表达。采用SPSS 16.0 统计软件对数据进行分析。结果 （1）低浓度的葡萄糖对MG63细胞增殖及其他生物学活性无显著影响；随着葡萄糖浓度的增高，其能够浓度依赖性抑制MG63细胞的增殖。（2）葡萄糖以浓度依赖方式影响细胞周期，高浓度葡萄糖可明显将细胞阻滞在G1期，S期细胞差异不显著，G2期细胞显著减少。（3）葡萄糖对 MG63细胞中TRAIL和OPGL mRNA表达的作用随浓度增加依次递增（P < 0. 05），而对OPG mRNA表达的作用随浓度增加依次递减（P < 0. 05）。结论 高糖环境可抑制MG63细胞增殖，可能导致成骨细胞TRAIL和OPGL表达增多、 OPG表达减少。

关键词: 高糖, MG63细胞, 细胞周期, 肿瘤坏死因子相关凋亡诱导配体