Abstract: Objective　To study the expression of miR-424 in ameloblastoma（AB）and its influence on the biological activity of AB. Methods　The qRT-PCR was used to detect the expression of miR-424 in AB and normal oral mucosa（NOM）tissues. After miR-424 mimics/negative control mimics were transfected into AM-1 cells，the influence of miR-424 on the proliferation，cell viability，and migration of AM-1 cells were examined using EdU，CCK-8，and scratch assays，respectively. Results　The relative expression of miR-424 in AB tissues was significantly lower than that in NOM tissues，and the difference was statistically significant（P < 0.05）. The miR-424 was able to significantly the proliferation ability of AM-1 cells（P < 0.05），and affect the activity of AM-1 cells（P < 0.05）. In addition，the total comparison of scratch area and scratch width between the negative control group and the experimental group showed statistically significant differences，and the differences became more obvious as the culture time increased（P < 0.05）. Conclusion　The miR-424 can significantly decrease the biological activity of AB and plays as a tumor suppressor in AB.

Key words: miR-424；ameloblastoma, AB；biological activity

摘要： 目的　研究miR-424在成釉细胞瘤（ameloblastoma，AB）中的表达情况及其对AB生物学活性的影响。方法　采用实时荧光定量PCR（qRT-PCR）检测AB和正常口腔黏膜（NOM）组织中miR-424的表达水平。将miR-424 mimics质粒、miR-424 negative control mimics质粒转染至人AB细胞系（AM-1）中，分别记为实验组和阴性对照组。采用EdU免疫荧光法、CCK-8法和划痕实验分别检测miR-424对AM-1细胞增殖能力、细胞活性和迁移能力的影响。结果　miR-424在AB组织中的相对表达量明显低于NOM组织，差异具有统计学意义（P <0.05）。miR-424能够显著降低AM-1细胞增殖能力，并能够影响AM-1的细胞活性（均P < 0.05）。此外，实验组与阴性对照组的划痕面积和宽度比较，差异具有统计学意义，且其差异随时间的延长而增大（均P < 0.05）。结论　miR-424能够显著降低AB的生物学活性，可能在AB中发挥抑癌基因作用。

关键词: miR-424, 成釉细胞瘤, 生物学活性